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A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis

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dc.contributor.author Ngamsom, B
dc.contributor.author Truyts, Alma E
dc.contributor.author Fourie, Louis J
dc.contributor.author Kumar, Shavon
dc.contributor.author Iles, A
dc.contributor.author Moodley, Klariska
dc.contributor.author Land, Kevin J
dc.contributor.author Pamme, N
dc.contributor.author Tarn, MD
dc.date.accessioned 2017-11-22T12:26:14Z
dc.date.available 2017-11-22T12:26:14Z
dc.date.issued 2017-08
dc.identifier.citation Ngamsom, B. et al. 2017. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry - A European Journal, vol. 23: DOI: 10.1002/chem.201703487 en_US
dc.identifier.issn 0947-6539
dc.identifier.uri http://onlinelibrary.wiley.com/doi/10.1002/chem.201703487/full
dc.identifier.uri DOI: 10.1002/chem.201703487
dc.identifier.uri http://hdl.handle.net/10204/9814
dc.description Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. en_US
dc.description.abstract We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(sup4) CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring. en_US
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.ispartofseries Worklist;19411
dc.subject Microfluidic systems en_US
dc.subject Escherichia coli en_US
dc.subject Immunomagnetic separation en_US
dc.subject IMS en_US
dc.title A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis en_US
dc.type Article en_US
dc.identifier.apacitation Ngamsom, B., Truyts, A. E., Fourie, L. J., Kumar, S., Iles, A., Moodley, K., ... Tarn, M. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/9814 en_ZA
dc.identifier.chicagocitation Ngamsom, B, Alma E Truyts, Louis J Fourie, Shavon Kumar, A Iles, Klariska Moodley, Kevin J Land, N Pamme, and MD Tarn "A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis." (2017) http://hdl.handle.net/10204/9814 en_ZA
dc.identifier.vancouvercitation Ngamsom B, Truyts AE, Fourie LJ, Kumar S, Iles A, Moodley K, et al. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. 2017; http://hdl.handle.net/10204/9814. en_ZA
dc.identifier.ris TY - Article AU - Ngamsom, B AU - Truyts, Alma E AU - Fourie, Louis J AU - Kumar, Shavon AU - Iles, A AU - Moodley, Klariska AU - Land, Kevin J AU - Pamme, N AU - Tarn, MD AB - We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(sup4) CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring. DA - 2017-08 DB - ResearchSpace DP - CSIR KW - Microfluidic systems KW - Escherichia coli KW - Immunomagnetic separation KW - IMS LK - https://researchspace.csir.co.za PY - 2017 SM - 0947-6539 T1 - A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis TI - A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis UR - http://hdl.handle.net/10204/9814 ER - en_ZA


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