We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1mL spiked buffer within 20min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10(sup4) CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.
Reference:
Ngamsom, B. et al. 2017. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry - A European Journal, vol. 23: DOI: 10.1002/chem.201703487
Ngamsom, B., Truyts, A. E., Fourie, L. J., Kumar, S., Iles, A., Moodley, K., ... Tarn, M. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/9814
Ngamsom, B, Alma E Truyts, Louis J Fourie, Shavon Kumar, A Iles, Klariska Moodley, Kevin J Land, N Pamme, and MD Tarn "A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis." (2017) http://hdl.handle.net/10204/9814
Ngamsom B, Truyts AE, Fourie LJ, Kumar S, Iles A, Moodley K, et al. A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. 2017; http://hdl.handle.net/10204/9814.
Copyright: 2017 Wiley. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.
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