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Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis

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dc.contributor.author Ngamsom, B
dc.contributor.author Fourie, L
dc.contributor.author Tarn, MD
dc.contributor.author Kumar, S
dc.contributor.author Moodley, K
dc.contributor.author Land, K
dc.contributor.author Pamme, N
dc.date.accessioned 2017-04-10T10:32:36Z
dc.date.available 2017-04-10T10:32:36Z
dc.date.issued 2016-10
dc.identifier.citation Ngamsom, B., Fourie, L., Tarn, M.D., Kumar, S., Moodley, K., Land, K. and Pamme, N. 2016. Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. In: Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, Ireland en_US
dc.identifier.isbn 978-0-9798064-9-0
dc.identifier.uri http://hdl.handle.net/10204/8990
dc.description Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, Ireland en_US
dc.description.abstract The present investigation reports isolation and detection of E. coli O157:H7 employing a simple and portable microfluidic device based on immiscible filtration assisted by surface tension (IFAST) and adenosine triphosphate (ATP) bioluminescence assay. The device demonstrated ca. 90% E. coli isolation with linear responses of bioluminescence signals from isolated cells at 6 – 600 CFU mL-1, suggesting great potential for point-of-need pathogenic bacteria detection for water analysis. en_US
dc.language.iso en en_US
dc.relation.ispartofseries Workflow;17850
dc.subject E. coli detection en_US
dc.subject Water analysis en_US
dc.subject Immiscible filtration assisted by surface tension en_US
dc.subject IFAST en_US
dc.subject Adenosine triphosphate en_US
dc.subject ATP en_US
dc.subject Microfluidics en_US
dc.subject Microsystem en_US
dc.subject Pathogen detection in water en_US
dc.title Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis en_US
dc.type Conference Presentation en_US
dc.identifier.apacitation Ngamsom, B., Fourie, L., Tarn, M., Kumar, S., Moodley, K., Land, K., & Pamme, N. (2016). Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/8990 en_ZA
dc.identifier.chicagocitation Ngamsom, B, L Fourie, MD Tarn, S Kumar, K Moodley, K Land, and N Pamme. "Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis." (2016): http://hdl.handle.net/10204/8990 en_ZA
dc.identifier.vancouvercitation Ngamsom B, Fourie L, Tarn M, Kumar S, Moodley K, Land K, et al, Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis; 2016. http://hdl.handle.net/10204/8990 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Ngamsom, B AU - Fourie, L AU - Tarn, MD AU - Kumar, S AU - Moodley, K AU - Land, K AU - Pamme, N AB - The present investigation reports isolation and detection of E. coli O157:H7 employing a simple and portable microfluidic device based on immiscible filtration assisted by surface tension (IFAST) and adenosine triphosphate (ATP) bioluminescence assay. The device demonstrated ca. 90% E. coli isolation with linear responses of bioluminescence signals from isolated cells at 6 – 600 CFU mL-1, suggesting great potential for point-of-need pathogenic bacteria detection for water analysis. DA - 2016-10 DB - ResearchSpace DP - CSIR KW - E. coli detection KW - Water analysis KW - Immiscible filtration assisted by surface tension KW - IFAST KW - Adenosine triphosphate KW - ATP KW - Microfluidics KW - Microsystem KW - Pathogen detection in water LK - https://researchspace.csir.co.za PY - 2016 SM - 978-0-9798064-9-0 T1 - Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis TI - Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis UR - http://hdl.handle.net/10204/8990 ER - en_ZA


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