The present investigation reports isolation and detection of E. coli O157:H7 employing a simple and portable microfluidic device based on immiscible filtration assisted by surface tension (IFAST) and adenosine triphosphate (ATP) bioluminescence assay. The device demonstrated ca. 90% E. coli isolation with linear responses of bioluminescence signals from isolated cells at 6 – 600 CFU mL-1, suggesting great potential for point-of-need pathogenic bacteria detection for water analysis.
Reference:
Ngamsom, B., Fourie, L., Tarn, M.D., Kumar, S., Moodley, K., Land, K. and Pamme, N. 2016. Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. In: Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, Ireland
Ngamsom, B., Fourie, L., Tarn, M., Kumar, S., Moodley, K., Land, K., & Pamme, N. (2016). Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/8990
Ngamsom, B, L Fourie, MD Tarn, S Kumar, K Moodley, K Land, and N Pamme. "Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis." (2016): http://hdl.handle.net/10204/8990
Ngamsom B, Fourie L, Tarn M, Kumar S, Moodley K, Land K, et al, Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis; 2016. http://hdl.handle.net/10204/8990 .
Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, Ireland
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