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Development of insect cell line using CRISPR technology

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dc.contributor.author Nweke, EE
dc.contributor.author Thimiri Govindaraj, Deepak B
dc.date.accessioned 2021-11-26T07:47:31Z
dc.date.available 2021-11-26T07:47:31Z
dc.date.issued 2021-02
dc.identifier.citation Nweke, E. & Thimiri Govindaraj, D.B. 2021. Development of insect cell line using CRISPR technology. <i>Progress in Molecular Biology and Translational Science, 180.</i> http://hdl.handle.net/10204/12174 en_ZA
dc.identifier.issn 1877-1173
dc.identifier.uri https://doi.org/10.1016/bs.pmbts.2021.01.003
dc.identifier.uri http://hdl.handle.net/10204/12174
dc.description.abstract In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases. en_US
dc.format Abstract en_US
dc.language.iso en en_US
dc.relation.uri https://www.sciencedirect.com/science/article/abs/pii/S1877117321000144 en_US
dc.source Progress in Molecular Biology and Translational Science, 180 en_US
dc.subject CRISPR/Cas9 en_US
dc.subject Drosophila en_US
dc.subject Spodoptera frugiperda en_US
dc.subject Sf9 en_US
dc.subject Sf21 en_US
dc.subject Synthetic baculoviral genome en_US
dc.subject SynBac en_US
dc.subject Insect cell lines en_US
dc.title Development of insect cell line using CRISPR technology en_US
dc.type Article en_US
dc.description.pages 1-20 en_US
dc.description.note Copyright: 2021 Elsevier Inc. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://www.sciencedirect.com/science/article/abs/pii/S1877117321000144 en_US
dc.description.cluster Next Generation Health en_US
dc.description.impactarea Synthetic Nanobiotech Biomachs en_US
dc.identifier.apacitation Nweke, E., & Thimiri Govindaraj, D. B. (2021). Development of insect cell line using CRISPR technology. <i>Progress in Molecular Biology and Translational Science, 180</i>, http://hdl.handle.net/10204/12174 en_ZA
dc.identifier.chicagocitation Nweke, EE, and Deepak B Thimiri Govindaraj "Development of insect cell line using CRISPR technology." <i>Progress in Molecular Biology and Translational Science, 180</i> (2021) http://hdl.handle.net/10204/12174 en_ZA
dc.identifier.vancouvercitation Nweke E, Thimiri Govindaraj DB. Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180. 2021; http://hdl.handle.net/10204/12174. en_ZA
dc.identifier.ris TY - Article AU - Nweke, EE AU - Thimiri Govindaraj, Deepak B AB - In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases. DA - 2021-02 DB - ResearchSpace DP - CSIR J1 - Progress in Molecular Biology and Translational Science, 180 KW - CRISPR/Cas9 KW - Drosophila KW - Spodoptera frugiperda KW - Sf9 KW - Sf21 KW - Synthetic baculoviral genome KW - SynBac KW - Insect cell lines LK - https://researchspace.csir.co.za PY - 2021 SM - 1877-1173 T1 - Development of insect cell line using CRISPR technology TI - Development of insect cell line using CRISPR technology UR - http://hdl.handle.net/10204/12174 ER - en_ZA
dc.identifier.worklist 25006 en_US


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