In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases.
Reference:
Nweke, E. & Thimiri Govindaraj, D.B. 2021. Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180. http://hdl.handle.net/10204/12174
Nweke, E., & Thimiri Govindaraj, D. B. (2021). Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180, http://hdl.handle.net/10204/12174
Nweke, EE, and Deepak B Thimiri Govindaraj "Development of insect cell line using CRISPR technology." Progress in Molecular Biology and Translational Science, 180 (2021) http://hdl.handle.net/10204/12174
Nweke E, Thimiri Govindaraj DB. Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180. 2021; http://hdl.handle.net/10204/12174.
