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Browsing by Author "O'Kennedy, Maretha M"

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The African perspective I: Using the new biosciences to support the African development agenda

Author: Morris, EJ ; Erasmus, C ; O'Kennedy, Maretha M Date: Sep 2016 Biopharmaceutical proteins and vaccines are traditionally produced in bacteria, eggs, yeast and animal cell cultures and are well established industries. More recently, these molecules are produced in plants and became known as biopharming. ... Read more
Biolistic mediated sorghum (Sorghum bicolor L. Moench) transformation via mannose and bialaphos based selection systems

Author: Grootboom, AW ; Mkhonza, NL ; O'Kennedy, Maretha M ; Chakauya, E ; Kunert, K ; Chikwamba, Rachel K Date: 2010 Sorghum (Sorghum bicolor (L.) Moench) is recalcitrant to transformation and besides several reported cases of success, transformation is far from routine. The choice of the selectable markers is key factor influencing success and efficiency ... Read more
Biolistic-mediated transformation protocols for maize and pearl millet using pre-cultured immature zygotic embryos and embryogenic tissue

Author: O'Kennedy, Maretha M ; Stark, HC ; Dube, N Date: Jan 2011 Maize (Zea mays L.) is the most important cereal food crop in sub-Saharan Africa and Latin America, and a key feed crop in Asia, whereas pearl millet [Pennisetum glaucum (L.) R. Br.] is a staple food that supplies a major proportion of calories ... Read more
Co-suppression of synthesis of major x-kafirin sub-class together with y-kafirin-1 and y-kafirin-2 required for substantially improved protein digestibility in transgenic sorghum

Author: Grootboom, AW ; Mkhonza, NL ; Mbambo, Z ; O'Kennedy, Maretha M ; Da Silva, LS ; Taylor, J ; Taylor, JRN ; Chikwamba, Rachel K ; Mehlo, L Date: Jan 2014 Co-suppressing major kafirin sub-classes is fundamental to improved protein digestibility and nutritional value of sorghum. The improvement is linked to an irregularly invaginated phenotype of protein bodies. Read more
Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens

Author: Abolnik, C ; Smith, Tanya ; Wandrag, BDR ; Murphy, M ; Rautenbach, M ; Olibile, O ; O'Kennedy, Maretha M Date: Jun 2022 Avian influenza poses one of the largest known threats to global poultry production and human health, but effective poultry vaccines can reduce infections rates, production losses and prevent mortalities, and reduce viral shed to limit further ... Read more
Efficacy of a plant-produced virus-like particle vaccine in chickens challenged with Influenza a H6N2 virus

Author: Smith, Tanya ; O'Kennedy, Maretha M ; Wandrag, Daniel BR ; Adeyemi, M ; Abolnik, C Date: Jul 2019 The efficacy, safety, speed, scalability, and cost-effectiveness of producing hemagglutinin-based virus-like particle (VLP) vaccines in plants is well-established for human influenza, but untested for the massive poultry influenza vaccine ... Read more
Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench

Author: Oldach, KH ; Morgenstern, A ; Rother, S ; Girgi, M ; O'Kennedy, Maretha M ; Lorz, H Date: Jul 2001 The authors report an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated ... Read more
Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet

Author: O'Kennedy, Maretha M ; Crampton, BG ; Lorito, M ; Chakauya, E ; Breese, WA ; Burger, JT ; Botha, FC Date: May 2011 Sclerospora graminicola is an Oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this ... Read more
Harnessing sorghum and millet biotechnology for food and health

Author: O'Kennedy, Maretha M ; Grootboom, A ; Shewry, PR Date: Nov 2006 This review highlights recombinant DNA technology as a powerful tool to enhance the gene pools of sorghum and pearl millet crops regarded as jewels of Africa. Although important advances in the improvement of these species have been made by ... Read more
An investigation into robust spectral indices for leaf chlorophyll estimation

Author: Main, Russell S ; Cho, Moses A ; Mathieu, Renaud SA ; O'Kennedy, Maretha M ; Ramoelo, Abel ; Koch, S Date: Nov 2011 Quantifying photosynthetic activity at the regional scale can provide important information to resource managers, planners and global ecosystem modelling efforts. With increasing availability of both hyperspectral and narrow band multispectral ... Read more
Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase

Author: O'Kennedy, Maretha M ; Burger, JT ; Botha, FC Date: Apr 2004 Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic ... Read more
Physiological responses of selected African sorghum landraces to progressive water stress and re-watering

Author: Devnarain, N ; Crampton, BG ; Chikwamba, Rachel K ; Becker, JVW ; O'Kennedy, Maretha M Date: Mar 2016 Sorghum is particularly drought tolerant compared with other cereal crops and is favoured for subsistence farming in water scarce regions of the world. This study was conducted to identify South African sorghum landraces with superior drought ... Read more
Protective immunity of plant-produced African horse sickness virus serotype 5 chimaeric virus-like particles (VLPs) and viral protein 2 (VP2) vaccines in IFNAR-/- mice

Author: O'Kennedy, Maretha M ; Coetzee, P ; Koekemoer, O ; Du Plessis, L ; Lourens, CW ; Kwezi, Lusisizwe ; Du Preez, Ilse ; Mamputha, Sipho ; Rutkowska, Daria A ; Lemmer, Yolandy Date: Aug 2022 Next generation vaccines have the capability to contribute to and revolutionise the veterinary vaccine industry. African horse sickness (AHS) is caused by an arbovirus infection and is characterised by respiratory distress and/or cardiovascular ... Read more
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses

Author: Dennis, SJ ; O'Kennedy, Maretha M ; Rutkowska, Daria A ; Tsekoa, Tsepo L ; Lourens, Carina W ; Hitzeroth, II ; Meyers, AE ; Rybicki, EP Date: Oct 2018 African horse sickness (AHS) is caused by multiple serotypes of the dsRNA AHSV and is a major scourge of domestic equids in Africa. While there are well established commercial live attenuated vaccines produced in South Africa, risks associated ... Read more
Sorghum biotechnology for food and health

Author: Mehlo, L ; Grootboom, M ; O'Kennedy, Maretha M ; Okole, B Date: Feb 2006 The goal of this project is to develop transgenic sorghum varieties that will deliver essential amino acids (lysine, threonine and tryptophan), vitamins A and E, iron and zinc - all of which are deficient in sorghum - to African populations ... Read more
Stable transformation of Hi-II maize using the particle inflow gun

Author: O'Kennedy, Maretha M ; Burger, JT ; Watson, TG Date: Apr 1998 Focuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture ... Read more
Transcriptomic analysis of a Sorghum bicolor landrace identifies a role for beta-alanine betaine biosynthesis in drought tolerance

Author: Devnarain, A ; Crampton, BG ; Olivier, N ; Van der Westhuyzen, Christiaan W ; Becker, JVW ; O'Kennedy, Maretha M Date: Dec 2019 Sorghum is indigenous to Africa and a remarkably drought tolerant cereal crop. In this study, the genetic response mechanisms involved in sorghum's tolerance to progressive water deficit and re-watering were investigated in a South African ... Read more
Transformation of elite white maize using the particle inflow gun and detailed analysis of a low-copy integration event

Author: O'Kennedy, Maretha M ; Burger, JT ; Berger, DK Date: Dec 2001 Elite white maize lines W506 and M37W were transformed with a selectable marker gene (bar) and a reporter gene (uidA) or the polygalacturonase-inhibiting protein (pgip) gene after bombardment of cultured immature zygotic embryos using the ... Read more
Transgenic and herbicide resistant pearl millet (Pennisetum glaucum L.) R.Br. via microprojectile bombardment of scutellar tissue

Author: Girgi, M ; O'Kennedy, Maretha M ; Morgenstern, A ; Mayer, G ; Lorz, H ; Oldach, KH Date: 2002 Four different pearl millet breeding lines were transformed and led to the regeneration of fertile transgenic plants. Scutellar tissue was bombarded with two plasmids containing the bar selectable marker and the beta-glucuronidase reporter ... Read more

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