Focuses on the transformation of the embryogenic type II callus, initiated from cultured immature zygotic embryos of the maize line Hi-II, by microprojectile bombardment using a particle inflow gun. Use of the plasmid pAHC25; Tissue culture and plant growth conditions; Optimization of bombardment conditions by transient expression; Southern blot analysis.
Reference:
O'Kennedy, MM, Burger, JT and Watson, TG. 1998. Stable transformation of Hi-II maize using the particle inflow gun. South African Journal of Science, Vol. South African Journal of Science 94(4), pp 188-192
O'Kennedy, M. M., Burger, J., & Watson, T. (1998). Stable transformation of Hi-II maize using the particle inflow gun. http://hdl.handle.net/10204/812
O'Kennedy, Maretha M, JT Burger, and TG Watson "Stable transformation of Hi-II maize using the particle inflow gun." (1998) http://hdl.handle.net/10204/812
O'Kennedy MM, Burger J, Watson T. Stable transformation of Hi-II maize using the particle inflow gun. 1998; http://hdl.handle.net/10204/812.
