dc.contributor.author |
Shibayama, Y
|
|
dc.contributor.author |
Fanucchi, Stephanie
|
|
dc.contributor.author |
Mhlanga, Musa
|
|
dc.date.accessioned |
2018-01-04T10:43:43Z |
|
dc.date.available |
2018-01-04T10:43:43Z |
|
dc.date.issued |
2017 |
|
dc.identifier.citation |
Shibayama, Y., Fanucchi, S. and Mhlanga, M.M. 2017. Visualization of enhancer-derived noncoding RNA. Enhancer RNAs: Methods and Protocols, vol. 1468: 19-32 |
en_US |
dc.identifier.isbn |
978-1-4939-4035-6 |
|
dc.identifier.uri |
DOI 10.1007/978-1-4939-4035-6_3
|
|
dc.identifier.uri |
http://www.springer.com/gp/book/9781493940332
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/9907
|
|
dc.description |
Copyright: 2017 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. |
en_US |
dc.description.abstract |
Enhancers are principal regulators that allow spatiotemporal tissue-specifi c control of gene expression. While mounting evidence suggests that enhancer-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via imaging techniques. This protocol describes a method to image eRNA in single cells by in situ hybridization followed by tyramide signal amplifi cation (TSA). The procedure can be multiplexed to simultaneously visualize both eRNA and protein-coding transcript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Springer |
en_US |
dc.relation.ispartofseries |
Worklist;20006 |
|
dc.subject |
RNA visualization |
en_US |
dc.subject |
Fluorescence in situ hybridization |
en_US |
dc.subject |
Tyramide signal amplification |
en_US |
dc.subject |
Enhancers |
en_US |
dc.subject |
Long ncRNA |
en_US |
dc.subject |
eRNA |
en_US |
dc.subject |
Single cell analysis |
en_US |
dc.title |
Visualization of enhancer-derived noncoding RNA |
en_US |
dc.type |
Book Chapter |
en_US |
dc.identifier.apacitation |
Shibayama, Y., Fanucchi, S., & Mhlanga, M. (2017). Visualization of enhancer-Derived noncoding RNA., <i>Worklist;20006</i> Springer. http://hdl.handle.net/10204/9907 |
en_ZA |
dc.identifier.chicagocitation |
Shibayama, Y, Stephanie Fanucchi, and Musa Mhlanga. "Visualization of enhancer-derived noncoding RNA" In <i>WORKLIST;20006</i>, n.p.: Springer. 2017. http://hdl.handle.net/10204/9907. |
en_ZA |
dc.identifier.vancouvercitation |
Shibayama Y, Fanucchi S, Mhlanga M. Visualization of enhancer-derived noncoding RNA.. Worklist;20006. [place unknown]: Springer; 2017. [cited yyyy month dd]. http://hdl.handle.net/10204/9907. |
en_ZA |
dc.identifier.ris |
TY - Book Chapter
AU - Shibayama, Y
AU - Fanucchi, Stephanie
AU - Mhlanga, Musa
AB - Enhancers are principal regulators that allow spatiotemporal tissue-specifi c control of gene expression. While mounting evidence suggests that enhancer-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via imaging techniques. This protocol describes a method to image eRNA in single cells by in situ hybridization followed by tyramide signal amplifi cation (TSA). The procedure can be multiplexed to simultaneously visualize both eRNA and protein-coding transcript at the site of transcriptional elongation, thereby permitting analysis of dynamics between the two transcript species in single cells. Our approach is not limited to eRNAs, but can be implemented on other transcripts.
DA - 2017
DB - ResearchSpace
DP - CSIR
KW - RNA visualization
KW - Fluorescence in situ hybridization
KW - Tyramide signal amplification
KW - Enhancers
KW - Long ncRNA
KW - eRNA
KW - Single cell analysis
LK - https://researchspace.csir.co.za
PY - 2017
SM - 978-1-4939-4035-6
T1 - Visualization of enhancer-derived noncoding RNA
TI - Visualization of enhancer-derived noncoding RNA
UR - http://hdl.handle.net/10204/9907
ER -
|
en_ZA |