dc.contributor.author |
Maphanga, Charles P
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dc.contributor.author |
Ombinda-Lemboumba, Saturnin
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dc.contributor.author |
Manoto, Sello L
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dc.contributor.author |
Maaza, Malik
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dc.contributor.author |
Mthunzi-Kufa, Patience
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dc.date.accessioned |
2017-09-19T08:31:41Z |
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dc.date.available |
2017-09-19T08:31:41Z |
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dc.date.issued |
2017-01 |
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dc.identifier.citation |
Maphanga, C.P., Ombinda-Lemboumba, S., Manoto, S.L., Maaza, M. and Mthunzi-Kufa, P. 2017. Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies. In: Proceedings of SPIE 10062, Optical Interactions with Tissue and Cells XXVIIISan Francisco, California, USA, 26 January - 03 February 2017 |
en_US |
dc.identifier.uri |
http://spie.org/Publications/Proceedings/Paper/10.1117/12.2252291?origin_id=x4323&start_year=1963
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dc.identifier.uri |
http://hdl.handle.net/10204/9581
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dc.description |
Proceedings of SPIE 10062, Optical Interactions with Tissue and Cells XXVIIISan Francisco, California, USA, 26 January - 03 February 2017. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website |
en_US |
dc.description.abstract |
Human immunodeficiency virus (HIV-1) infection still remains one amongst the world’s most challenging infections since its discovery. Antiretroviral therapy is the recommended treatment of choice for HIV-1 infection taken by patients orally. The highly active antiretroviral therapy (HAART) prevents the replication of HIV-1 and further destruction of the immune system, therefore enabling the body to fight opportunistic life-threatening infections, cancers, and also arrest HIV infection from advancing to AIDS. The major challenge with HAART is the inability to reach the viral reservoirs where the HIV-1 remains latent and persistent, leading to inability to fully eradicate the virus. This study is aimed at initially designing and assembling a fully functional optical translocation setup to optically deliver antiretroviral drugs into HIV-1 infected cells in a targeted manner using Gaussian beam mode femtosecond laser pulses in-vitro. The main objective of our study is to define the in-vitro drug photo-translocation parameters to allow future design of an efficient drug delivery device with potential in-vivo drug delivery applications. In our experiments, HEK 293T cells were used to produce HIV-1 enveloped pseudovirus (ZM53) to infect TZM-bl cells which were later treated with laser pulses emitted by a titanium sapphire laser (800 nm, 1KHz, 113 fs, ~ 6.5 µW) to create sub-microscopic pores on the cell membrane enabling influx of extracellular media. Following laser treatment, changes in cellular responses were analysed using cell morphology studies, cytotoxicity, and luciferase assay studies. Controls included laser untreated cells incubated with the drug for 72 hours. The data in this study was statistically analysed using the SigmaPlot software version 13. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
SPIE |
en_US |
dc.relation.ispartofseries |
Workflow;18700 |
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dc.subject |
Human Immunodeficiency Virus |
en_US |
dc.subject |
HIV |
en_US |
dc.subject |
Highly Active Antiretroviral Therapy |
en_US |
dc.subject |
HAART |
en_US |
dc.subject |
Acquired Immunodeficiency Syndrome |
en_US |
dc.subject |
AIDS |
en_US |
dc.subject |
Gaussian beams |
en_US |
dc.subject |
Femtosecond pulses |
en_US |
dc.subject |
Optical translocation |
en_US |
dc.subject |
TZM-bl cells |
en_US |
dc.subject |
Sub-microscopic pores |
en_US |
dc.subject |
Cell morphology |
en_US |
dc.subject |
Cell cytotoxicity |
en_US |
dc.subject |
Targeted drug delivery |
en_US |
dc.title |
Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper] |
en_US |
dc.type |
Conference Presentation |
en_US |
dc.identifier.apacitation |
Maphanga, C. P., Ombinda-Lemboumba, S., Manoto, S. L., Maaza, M., & Mthunzi-Kufa, P. (2017). Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper]. SPIE. http://hdl.handle.net/10204/9581 |
en_ZA |
dc.identifier.chicagocitation |
Maphanga, Charles P, Saturnin Ombinda-Lemboumba, Sello L Manoto, Malik Maaza, and Patience Mthunzi-Kufa. "Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper]." (2017): http://hdl.handle.net/10204/9581 |
en_ZA |
dc.identifier.vancouvercitation |
Maphanga CP, Ombinda-Lemboumba S, Manoto SL, Maaza M, Mthunzi-Kufa P, Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper]; SPIE; 2017. http://hdl.handle.net/10204/9581 . |
en_ZA |
dc.identifier.ris |
TY - Conference Presentation
AU - Maphanga, Charles P
AU - Ombinda-Lemboumba, Saturnin
AU - Manoto, Sello L
AU - Maaza, Malik
AU - Mthunzi-Kufa, Patience
AB - Human immunodeficiency virus (HIV-1) infection still remains one amongst the world’s most challenging infections since its discovery. Antiretroviral therapy is the recommended treatment of choice for HIV-1 infection taken by patients orally. The highly active antiretroviral therapy (HAART) prevents the replication of HIV-1 and further destruction of the immune system, therefore enabling the body to fight opportunistic life-threatening infections, cancers, and also arrest HIV infection from advancing to AIDS. The major challenge with HAART is the inability to reach the viral reservoirs where the HIV-1 remains latent and persistent, leading to inability to fully eradicate the virus. This study is aimed at initially designing and assembling a fully functional optical translocation setup to optically deliver antiretroviral drugs into HIV-1 infected cells in a targeted manner using Gaussian beam mode femtosecond laser pulses in-vitro. The main objective of our study is to define the in-vitro drug photo-translocation parameters to allow future design of an efficient drug delivery device with potential in-vivo drug delivery applications. In our experiments, HEK 293T cells were used to produce HIV-1 enveloped pseudovirus (ZM53) to infect TZM-bl cells which were later treated with laser pulses emitted by a titanium sapphire laser (800 nm, 1KHz, 113 fs, ~ 6.5 µW) to create sub-microscopic pores on the cell membrane enabling influx of extracellular media. Following laser treatment, changes in cellular responses were analysed using cell morphology studies, cytotoxicity, and luciferase assay studies. Controls included laser untreated cells incubated with the drug for 72 hours. The data in this study was statistically analysed using the SigmaPlot software version 13.
DA - 2017-01
DB - ResearchSpace
DP - CSIR
KW - Human Immunodeficiency Virus
KW - HIV
KW - Highly Active Antiretroviral Therapy
KW - HAART
KW - Acquired Immunodeficiency Syndrome
KW - AIDS
KW - Gaussian beams
KW - Femtosecond pulses
KW - Optical translocation
KW - TZM-bl cells
KW - Sub-microscopic pores
KW - Cell morphology
KW - Cell cytotoxicity
KW - Targeted drug delivery
LK - https://researchspace.csir.co.za
PY - 2017
T1 - Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper]
TI - Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [conference paper]
UR - http://hdl.handle.net/10204/9581
ER -
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en_ZA |