Bidens pilosa is a medicinal plant used for the treatment of several physiological illnesses. In South Africa, as the case may be for other African countries, this plant is equally consumed as a vegetable. In the current study, pressurized hot water extraction (PHWE) technology was employed for the extraction of polyphenolic compounds from leaves of B. pilosa under two different temperature conditions (100 and 150 °C). Accordingly, extraction of these compounds was made possible at 150 °C and analysis of these extracts using UPLC-qTOF-MS/MS revealed the presence of several hydoxylcinnamoyl tartaric acids. Here, different isomers of coutaric-, caftaric-, fertaric-, chicoric acid and caftaric acid glycosides were detected. The contribution of mass spectrometry fragmentation towards the characterization of these molecules is also presented. To the best of our knowledge, this is the first report of these molecules in B. pilosa.
Reference:
Khoza, B.S., Gbashi, S., Steenkamp, P.A. et al. 2016. Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry. South African Journal of Botany, vol. 103: 95-100. https://doi.org/10.1016/j.sajb.2015.08.018
Khoza, B., Gbashi, S., Steenkamp, P. A., Njobe, P., & Madala, N. (2016). Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry. http://hdl.handle.net/10204/9530
Khoza, BS, S Gbashi, Paul A Steenkamp, PB Njobe, and NE Madala "Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry." (2016) http://hdl.handle.net/10204/9530
Khoza B, Gbashi S, Steenkamp PA, Njobe P, Madala N. Identification of hydroxylcinnamoyl tartaric acid esters in Bidens pilosa by UPLC-tandem mass spectrometry. 2016; http://hdl.handle.net/10204/9530.
Copyright: 2015 SAAB. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.