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Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid

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dc.contributor.author Mhlongo, MI
dc.contributor.author Piater, LA
dc.contributor.author Steenkamp, Paul A
dc.contributor.author Madala, NE
dc.contributor.author Dubery, IA
dc.date.accessioned 2017-07-28T09:11:31Z
dc.date.available 2017-07-28T09:11:31Z
dc.date.issued 2015-01
dc.identifier.citation Mhlongo, M.I., Piater, L.A., Steenkamp, P.A. et al. 2015. Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. Biotechnology Letters, vol. 37(1): 205-209. DOI: 10.1007/s10529-014-1645-8 en_US
dc.identifier.issn 0141-5492
dc.identifier.uri https://link.springer.com/content/pdf/10.1007/s10529-014-1645-8.pdf
dc.identifier.uri DOI: 10.1007/s10529-014-1645-8
dc.identifier.uri https://link.springer.com/article/10.1007/s10529-014-1645-8
dc.identifier.uri http://hdl.handle.net/10204/9390
dc.description Copyright: 2014 Springer. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. en_US
dc.description.abstract Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to sunlight. Very little, however, is known about the biological significance and origin of cis-isomers. Here we show for the first time the accumulation of cis-5-caffeoylquinic acid in cultured tobacco cells treated with different inducers of plant defence (lipopolysaccharides, flagellin peptide-22, chitosan, acibenzolar-Smethyl and isonitrosoacetophenone), without exposure to UV light and with a 2-fold (on average) increase in the concentration of the pool in comparison to non-stimulated cells. Our UHPLC–Q-TOF-MS and multivariate statistical results suggest the presence of a possible biological pathway responsible for the production of cis-CGAs in tobacco plants. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.relation.ispartofseries Worklist;17672
dc.subject Cis-5-caffeoylquinic acid en_US
dc.subject Chlorogenic acids en_US
dc.subject Natural products en_US
dc.subject Phytoanticipins en_US
dc.subject Plants en_US
dc.subject Secondary metabolism en_US
dc.subject Tobacco cells en_US
dc.title Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid en_US
dc.type Article en_US
dc.identifier.apacitation Mhlongo, M., Piater, L., Steenkamp, P. A., Madala, N., & Dubery, I. (2015). Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. http://hdl.handle.net/10204/9390 en_ZA
dc.identifier.chicagocitation Mhlongo, MI, LA Piater, Paul A Steenkamp, NE Madala, and IA Dubery "Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid." (2015) http://hdl.handle.net/10204/9390 en_ZA
dc.identifier.vancouvercitation Mhlongo M, Piater L, Steenkamp PA, Madala N, Dubery I. Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid. 2015; http://hdl.handle.net/10204/9390. en_ZA
dc.identifier.ris TY - Article AU - Mhlongo, MI AU - Piater, LA AU - Steenkamp, Paul A AU - Madala, NE AU - Dubery, IA AB - Previous studies suggest that only transisomers of chlorogenic acid (CGA) are naturally produced. Cis-isomers have been noted in some plant tissues exposed to different mechanical processes as well as untreated tobacco leaves exposed to sunlight. Very little, however, is known about the biological significance and origin of cis-isomers. Here we show for the first time the accumulation of cis-5-caffeoylquinic acid in cultured tobacco cells treated with different inducers of plant defence (lipopolysaccharides, flagellin peptide-22, chitosan, acibenzolar-Smethyl and isonitrosoacetophenone), without exposure to UV light and with a 2-fold (on average) increase in the concentration of the pool in comparison to non-stimulated cells. Our UHPLC–Q-TOF-MS and multivariate statistical results suggest the presence of a possible biological pathway responsible for the production of cis-CGAs in tobacco plants. DA - 2015-01 DB - ResearchSpace DP - CSIR KW - Cis-5-caffeoylquinic acid KW - Chlorogenic acids KW - Natural products KW - Phytoanticipins KW - Plants KW - Secondary metabolism KW - Tobacco cells LK - https://researchspace.csir.co.za PY - 2015 SM - 0141-5492 T1 - Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid TI - Metabolomic fingerprinting of primed tobacco cells provide the first evidence for the biological origin of cis-chlorogenic acid UR - http://hdl.handle.net/10204/9390 ER - en_ZA


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