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Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

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dc.contributor.author Makola, MM
dc.contributor.author Steenkamp, Paul A
dc.contributor.author Dubery, IA
dc.contributor.author Kabanda, MM
dc.contributor.author Madala, NE
dc.date.accessioned 2017-06-07T07:58:29Z
dc.date.available 2017-06-07T07:58:29Z
dc.date.issued 2016-03
dc.identifier.citation Makola, M.M., Steenkamp, P.A., Dubery, I.A. et al. 2016. Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry, vol. 30(8): 1011-1018. DOI: 10.1002/rcm.7526 en_US
dc.identifier.issn 1097-0231
dc.identifier.uri http://onlinelibrary.wiley.com/doi/10.1002/rcm.7526/abstract;jsessionid=E7960DC361B8098BEBC116C048CFFA72.f03t03
dc.identifier.uri DOI: 10.1002/rcm.7526
dc.identifier.uri http://hdl.handle.net/10204/9211
dc.description Copyright: 2016 Wiley. Due to copyright restrictions, the attached pdf contains the pre-print version of the accepted article. The definitive published version can be obtained via the publisher's website. en_US
dc.description.abstract Caffeoylquinic acid (CQA) derivatives are a group of structurally diverse phytochemicals that have attracted attention due to their many health benefits. The structural diversity of these molecules is due in part to the presence of regio- and geometrical isomerism. This structural diversity hampers the accurate annotation of these molecules in plant extracts. Mass spectrometry (MS) is successfully used to differentiate between the different regioisomers of the CQA derivatives; however, the accurate discrimination of the geometrical isomers of these molecules has proven to be an elusive task. UV-irradiated methanolic solutions of diCQA were analyzed using an ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOFMS) method in negative ionisation mode. An in-source collision-induced dissociation (ISCID) method was optimized by varying both the capillary and cone voltages to achieve differential fragmentation patterns between UV-generated geometrical isomers of the diCQAs during MS analyses. Changes in the capillary voltage did not cause a significant difference to the fragmentation patterns of the four geometrical isomers, while changes in the cone voltage resulted in significant differences in the fragmentation patterns. The results also show, for the first time, the preferential formation of alkali metal (Li+, Na+ and K+) adducts by the cis geometrical isomers of diCQAs, compared to their trans counterparts. Optimized QTOFMS-based methods may be used to differentiate the geometrical isomers of diCQAs. Finally, additives such as metal salts to induce adduct formation can be applied as an alternative method to differentiate closely related isomers which could have been difficult to differentiate under normal MS settings. en_US
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.ispartofseries Worklist;17674
dc.subject Caffeoylquinic acid en_US
dc.subject Phytochemicals en_US
dc.subject Isomerisms en_US
dc.title Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry en_US
dc.type Article en_US
dc.identifier.apacitation Makola, M., Steenkamp, P. A., Dubery, I., Kabanda, M., & Madala, N. (2016). Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. http://hdl.handle.net/10204/9211 en_ZA
dc.identifier.chicagocitation Makola, MM, Paul A Steenkamp, IA Dubery, MM Kabanda, and NE Madala "Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry." (2016) http://hdl.handle.net/10204/9211 en_ZA
dc.identifier.vancouvercitation Makola M, Steenkamp PA, Dubery I, Kabanda M, Madala N. Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. 2016; http://hdl.handle.net/10204/9211. en_ZA
dc.identifier.ris TY - Article AU - Makola, MM AU - Steenkamp, Paul A AU - Dubery, IA AU - Kabanda, MM AU - Madala, NE AB - Caffeoylquinic acid (CQA) derivatives are a group of structurally diverse phytochemicals that have attracted attention due to their many health benefits. The structural diversity of these molecules is due in part to the presence of regio- and geometrical isomerism. This structural diversity hampers the accurate annotation of these molecules in plant extracts. Mass spectrometry (MS) is successfully used to differentiate between the different regioisomers of the CQA derivatives; however, the accurate discrimination of the geometrical isomers of these molecules has proven to be an elusive task. UV-irradiated methanolic solutions of diCQA were analyzed using an ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOFMS) method in negative ionisation mode. An in-source collision-induced dissociation (ISCID) method was optimized by varying both the capillary and cone voltages to achieve differential fragmentation patterns between UV-generated geometrical isomers of the diCQAs during MS analyses. Changes in the capillary voltage did not cause a significant difference to the fragmentation patterns of the four geometrical isomers, while changes in the cone voltage resulted in significant differences in the fragmentation patterns. The results also show, for the first time, the preferential formation of alkali metal (Li+, Na+ and K+) adducts by the cis geometrical isomers of diCQAs, compared to their trans counterparts. Optimized QTOFMS-based methods may be used to differentiate the geometrical isomers of diCQAs. Finally, additives such as metal salts to induce adduct formation can be applied as an alternative method to differentiate closely related isomers which could have been difficult to differentiate under normal MS settings. DA - 2016-03 DB - ResearchSpace DP - CSIR KW - Caffeoylquinic acid KW - Phytochemicals KW - Isomerisms LK - https://researchspace.csir.co.za PY - 2016 SM - 1097-0231 T1 - Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry TI - Preferential alkali metal adduct formation by cis geometrical isomers of dicaffeoylquinic acids allows for efficient discrimination from their trans isomers during ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry UR - http://hdl.handle.net/10204/9211 ER - en_ZA


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