ResearchSpace

Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish

Show simple item record

dc.contributor.author Lalloo, Rajesh
dc.contributor.author Ramchuran, Santosh O
dc.contributor.author Ramduth, DM
dc.contributor.author Gorgens, J
dc.date.accessioned 2007-06-29T09:43:48Z
dc.date.available 2007-06-29T09:43:48Z
dc.date.issued 2007-02
dc.identifier.citation Lalloo, R et al. 2007. Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. Journal of Applied Microbiology, pp 1-9 en
dc.identifier.issn 1364-5072
dc.identifier.uri http://hdl.handle.net/10204/825
dc.identifier.uri https://pubmed.ncbi.nlm.nih.gov/17953558/
dc.identifier.uri DOI: 10.1111/j.1365-2672.2007.03360.x
dc.description Copyright: 2007 Blackwell Publishing en
dc.description.abstract Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibi-tion of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a pre-liminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products. en
dc.language.iso en en
dc.publisher Blackwell Publishing en
dc.subject Aquaculture en
dc.subject Bacillus spp en
dc.subject Bacterial pathogens en
dc.subject Biological agent en
dc.subject Water quality en
dc.subject Mud sediment en
dc.subject Cyprinus carpio en
dc.title Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish en
dc.type Article en
dc.identifier.apacitation Lalloo, R., Ramchuran, S. O., Ramduth, D., & Gorgens, J. (2007). Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. http://hdl.handle.net/10204/825 en_ZA
dc.identifier.chicagocitation Lalloo, Rajesh, Santosh O Ramchuran, DM Ramduth, and J Gorgens "Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish." (2007) http://hdl.handle.net/10204/825 en_ZA
dc.identifier.vancouvercitation Lalloo R, Ramchuran SO, Ramduth D, Gorgens J. Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. 2007; http://hdl.handle.net/10204/825. en_ZA
dc.identifier.ris TY - Article AU - Lalloo, Rajesh AU - Ramchuran, Santosh O AU - Ramduth, DM AU - Gorgens, J AB - Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibi-tion of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a pre-liminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products. DA - 2007-02 DB - ResearchSpace DP - CSIR KW - Aquaculture KW - Bacillus spp KW - Bacterial pathogens KW - Biological agent KW - Water quality KW - Mud sediment KW - Cyprinus carpio LK - https://researchspace.csir.co.za PY - 2007 SM - 1364-5072 T1 - Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish TI - Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish UR - http://hdl.handle.net/10204/825 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record