dc.contributor.author |
Lemmer, Yolandy
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dc.contributor.author |
Kalombo, Lonji
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dc.contributor.author |
Pietersen, R-D
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dc.contributor.author |
Jones, AT
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dc.contributor.author |
Semete-Makokotlela, Boitumelo
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dc.contributor.author |
Van Wyngaardt, S
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dc.contributor.author |
Ramalapa, BE
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dc.contributor.author |
Stoltz, A
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dc.contributor.author |
Baker, B
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dc.contributor.author |
Verschoor, JA
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dc.contributor.author |
Swai, HS
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dc.contributor.author |
De Chastellier, C
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dc.date.accessioned |
2015-10-05T07:22:46Z |
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dc.date.available |
2015-10-05T07:22:46Z |
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dc.date.issued |
2015-06 |
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dc.identifier.citation |
Lemmer, Y, Kalombo, L, Pietersen, R-D, Jones, A.T, Semete-Makokotlela, B, Van Wyngaardt, S, Ramalapa, B.E, Stoltz, A, Baker, B, Verschoor, J.A, Swai, H.S and De Chastellier, C. 2015. Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. Journal of Controlled Release, VOL. 211, pp 94-104 |
en_US |
dc.identifier.issn |
0168-3659 |
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dc.identifier.uri |
http://ac.els-cdn.com/S0168365915006124/1-s2.0-S0168365915006124-main.pdf?_tid=fa662424-5c7c-11e5-afed-00000aab0f6b&acdnat=1442412935_6485267f0df29d0a8c408a42418bcaf7
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dc.identifier.uri |
http://hdl.handle.net/10204/8148
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dc.description |
Copyright: 2015 Elsevier. This is a post-print version. The definitive version of the work is published in Journal of Controlled Release, vol. 211, pp 94-104 |
en_US |
dc.description.abstract |
The appearance of drug-resistant strains of Mycobacterium tuberculosis (Mtb) poses a great challenge to the development of novel treatment programmes to combat tuberculosis. Since innovative nanotechnologies might alleviate the limitations of current therapies, we have designed a new nanoformulation for use as an anti-TB drug delivery system. It consists of incorporating mycobacterial cellwall mycolic acids (MA) as targeting ligands into a drug-encapsulating Poly DL-lactic-co-glycolic acid polymer (PLGA), via a double emulsion solvent evaporation technique. Bone marrow-derived mouse macrophages, either uninfected or infected with different mycobacterial strains (Mycobacterium avium, Mycobacterium bovis BCG or Mtb), were exposed to encapsulated isoniazid-PLGA nanoparticles (NPs) using MA as a targeting ligand. The fate of the NPs was monitored by electron microscopy. Our study showed that i) the inclusion of MA in the nanoformulations resulted in their expression on the outer surface and a significant increase in phagocytic uptake of the NPs; ii) nanoparticle-containing phagosomes were rapidly processed into phagolysosomes, whether MA had been included or not; and iii) nanoparticle-containing phagolysosomes did not fuse with non-matured mycobacterium-containing phagosomes, but fusion events with mycobacterium-containing phagolysosomes were clearly observed. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.relation.ispartofseries |
Workflow;15450 |
|
dc.subject |
Tuberculosis |
en_US |
dc.subject |
Mycolic acids |
en_US |
dc.subject |
Nanodrug delivery |
en_US |
dc.subject |
Targeting |
en_US |
dc.subject |
Phagosomes |
en_US |
dc.subject |
Electron microscopy |
en_US |
dc.subject |
Mycobacterium tuberculosis |
en_US |
dc.subject |
Mtb |
en_US |
dc.title |
Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Lemmer, Y., Kalombo, L., Pietersen, R., Jones, A., Semete-Makokotlela, B., Van Wyngaardt, S., ... De Chastellier, C. (2015). Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. http://hdl.handle.net/10204/8148 |
en_ZA |
dc.identifier.chicagocitation |
Lemmer, Yolandy, Lonji Kalombo, R-D Pietersen, AT Jones, Boitumelo Semete-Makokotlela, S Van Wyngaardt, BE Ramalapa, et al "Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis." (2015) http://hdl.handle.net/10204/8148 |
en_ZA |
dc.identifier.vancouvercitation |
Lemmer Y, Kalombo L, Pietersen R, Jones A, Semete-Makokotlela B, Van Wyngaardt S, et al. Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. 2015; http://hdl.handle.net/10204/8148. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Lemmer, Yolandy
AU - Kalombo, Lonji
AU - Pietersen, R-D
AU - Jones, AT
AU - Semete-Makokotlela, Boitumelo
AU - Van Wyngaardt, S
AU - Ramalapa, BE
AU - Stoltz, A
AU - Baker, B
AU - Verschoor, JA
AU - Swai, HS
AU - De Chastellier, C
AB - The appearance of drug-resistant strains of Mycobacterium tuberculosis (Mtb) poses a great challenge to the development of novel treatment programmes to combat tuberculosis. Since innovative nanotechnologies might alleviate the limitations of current therapies, we have designed a new nanoformulation for use as an anti-TB drug delivery system. It consists of incorporating mycobacterial cellwall mycolic acids (MA) as targeting ligands into a drug-encapsulating Poly DL-lactic-co-glycolic acid polymer (PLGA), via a double emulsion solvent evaporation technique. Bone marrow-derived mouse macrophages, either uninfected or infected with different mycobacterial strains (Mycobacterium avium, Mycobacterium bovis BCG or Mtb), were exposed to encapsulated isoniazid-PLGA nanoparticles (NPs) using MA as a targeting ligand. The fate of the NPs was monitored by electron microscopy. Our study showed that i) the inclusion of MA in the nanoformulations resulted in their expression on the outer surface and a significant increase in phagocytic uptake of the NPs; ii) nanoparticle-containing phagosomes were rapidly processed into phagolysosomes, whether MA had been included or not; and iii) nanoparticle-containing phagolysosomes did not fuse with non-matured mycobacterium-containing phagosomes, but fusion events with mycobacterium-containing phagolysosomes were clearly observed.
DA - 2015-06
DB - ResearchSpace
DP - CSIR
KW - Tuberculosis
KW - Mycolic acids
KW - Nanodrug delivery
KW - Targeting
KW - Phagosomes
KW - Electron microscopy
KW - Mycobacterium tuberculosis
KW - Mtb
LK - https://researchspace.csir.co.za
PY - 2015
SM - 0168-3659
T1 - Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis
TI - Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis
UR - http://hdl.handle.net/10204/8148
ER -
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en_ZA |