ResearchSpace

Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species

Show simple item record

dc.contributor.author Madala, NE
dc.contributor.author Tugizimana, F
dc.contributor.author Steenkamp, PA
dc.date.accessioned 2015-08-19T10:53:10Z
dc.date.available 2015-08-19T10:53:10Z
dc.date.issued 2014-09
dc.identifier.citation Madala, NE, Tugizimana, F and Steenkamp, PA. 2014. Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. Journal of Analytical Methods in Chemistry, vol. 10, pp 1-8 en_US
dc.identifier.issn 2090-8865
dc.identifier.uri http://hdl.handle.net/10204/8062
dc.description Copyright: Hindawi Publishing Corporation 2014. en_US
dc.description.abstract Chlorogenic acids (CGA) have been profiled in the leaves of Momordica balsamina, Momordica charantia, and Momordica foetida. All three species were found to contain the trans and cis isomers of 4-acyl para-coumaroylquinic acid (pCoQA), caffeoylquinic acid (CQA), and feruloylquinic acid (FQA). To the best of our knowledge, this is the first report of pCoQA and FQA and their cis isomers in these Momordica species. These profiles were obtained by a newly developed UPLC-qTOF-MS method based on the in-source collision induced dissociation (ISCID) method optimized to mimic the MS (sup2) andMS(sup3) fragmentation of an ion trap-basedMS. The presence of the cis isomers is believed to be due to high UV exposure of these plants. Furthermore, the absence of the 3-acyl and 5-acyl CGA molecules points to a metabolic mark that is unusual and represents a very interesting biochemical phenotype of these species. Our optimized ISCID method was also shown to be able to distinguish between the geometrical isomers of all three forms of CGA, a phenomenon previously deemed impossible with other common mass spectrometry systems used for CGA analyses. en_US
dc.language.iso en en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.relation.ispartofseries Workflow;14635
dc.subject Chlorogenic acids en_US
dc.subject CGA en_US
dc.subject Momordica balsamina en_US
dc.subject Momordica charantia en_US
dc.subject Momordica foetida en_US
dc.title Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species en_US
dc.type Article en_US
dc.identifier.apacitation Madala, N., Tugizimana, F., & Steenkamp, P. (2014). Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. http://hdl.handle.net/10204/8062 en_ZA
dc.identifier.chicagocitation Madala, NE, F Tugizimana, and PA Steenkamp "Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species." (2014) http://hdl.handle.net/10204/8062 en_ZA
dc.identifier.vancouvercitation Madala N, Tugizimana F, Steenkamp P. Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. 2014; http://hdl.handle.net/10204/8062. en_ZA
dc.identifier.ris TY - Article AU - Madala, NE AU - Tugizimana, F AU - Steenkamp, PA AB - Chlorogenic acids (CGA) have been profiled in the leaves of Momordica balsamina, Momordica charantia, and Momordica foetida. All three species were found to contain the trans and cis isomers of 4-acyl para-coumaroylquinic acid (pCoQA), caffeoylquinic acid (CQA), and feruloylquinic acid (FQA). To the best of our knowledge, this is the first report of pCoQA and FQA and their cis isomers in these Momordica species. These profiles were obtained by a newly developed UPLC-qTOF-MS method based on the in-source collision induced dissociation (ISCID) method optimized to mimic the MS (sup2) andMS(sup3) fragmentation of an ion trap-basedMS. The presence of the cis isomers is believed to be due to high UV exposure of these plants. Furthermore, the absence of the 3-acyl and 5-acyl CGA molecules points to a metabolic mark that is unusual and represents a very interesting biochemical phenotype of these species. Our optimized ISCID method was also shown to be able to distinguish between the geometrical isomers of all three forms of CGA, a phenomenon previously deemed impossible with other common mass spectrometry systems used for CGA analyses. DA - 2014-09 DB - ResearchSpace DP - CSIR KW - Chlorogenic acids KW - CGA KW - Momordica balsamina KW - Momordica charantia KW - Momordica foetida LK - https://researchspace.csir.co.za PY - 2014 SM - 2090-8865 T1 - Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species TI - Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species UR - http://hdl.handle.net/10204/8062 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record