Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol.
Reference:
De Souza Gil, E, Rezende, S.G, Ribeiro Júnior, E.J.M, Barcelos, H.T, Scalize, P.S, Santiago, M.F, Quintino, M.P and Somerset, V.S. 2014. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters. In: Sensing in Electroanalysis, University Press Centre, Pardubice, Czech Republic, vol.8, pp 227-240
De Souza Gil, E., Rezende, S., Ribeiro Júnior, E., Barcelos, H., Scalize, P., Santiago, M., ... Somerset, V. (2014). Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters., Workflow;14109 University Press Centre. http://hdl.handle.net/10204/7936
De Souza Gil, E, SG Rezende, EJM Ribeiro Júnior, HT Barcelos, PS Scalize, MF Santiago, MP Quintino, and VS Somerset. "Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters" In WORKFLOW;14109, n.p.: University Press Centre. 2014. http://hdl.handle.net/10204/7936.
De Souza Gil E, Rezende S, Ribeiro Júnior E, Barcelos H, Scalize P, Santiago M, et al. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters.. Workflow;14109. [place unknown]: University Press Centre; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/7936.
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