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Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae

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dc.contributor.author Ssemakalu, CC
dc.contributor.author Le Roux, Wouter J
dc.contributor.author Pillay, M
dc.date.accessioned 2014-02-28T06:54:02Z
dc.date.available 2014-02-28T06:54:02Z
dc.date.issued 2013-09
dc.identifier.citation Ssemakalu, C.C, Le Roux, W.J. and Pillay, M. 2013. Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae. South African Journal of Science, vol. 109(9/10), pp 1-6 en_US
dc.identifier.issn 0038-2353
dc.identifier.uri http://www.sajs.co.za/sites/default/files/publications/pdf/Ssemakalu%20et%20al_Research%20Article.pdf
dc.identifier.uri http://hdl.handle.net/10204/7259
dc.description Copyright: 2013 AOSIS. This is an open access journal. The journal authorizes the publication of the information herewith contained. Published in South African Journal of Science, vol. 109(9/10), pp 1-6 en_US
dc.description.abstract Cholera toxin is the aetiological agent of cholera – a deadly waterborne disease acquired through the consumption of untreated water contaminated with CTX bacteriophage harbouring strains of V. cholerae. Solar disinfection is a re-emerging technique that relies on the ultraviolet component of sunlight to inactivate the growth of Vibrio cholerae in water, rendering the water microbiologically safe for consumption. However, studies have shown that DNA damaging agents, such as ultraviolet light, induce the replication of the CTX bacteriophage with subsequent expression of the cholera toxin. In this study we investigated the impact of solar irradiation on the secretion of cholera toxin by toxigenic strains of V. cholerae in water. The cholera toxin ELISA assay, qualitative and quantitative real-time PCR as well as growth on solid media were used to determine cholera toxin secretion, DNA integrity and growth of the bacteria after 7 h and 31 h of solar irradiation. Solar irradiation in water reduced the integrity of DNA, inactivated the growth of V. cholerae and, most importantly, prevented the secretion of detectable levels of cholera toxin. This finding is encouraging for resource-poor communities that may rely on solar disinfection to alleviate the burden of cholera-related fatalities. en_US
dc.language.iso en en_US
dc.publisher AOSIS en_US
dc.relation.ispartofseries Workflow;12186
dc.subject Solar disinfection en_US
dc.subject SODIS en_US
dc.subject Cholera en_US
dc.subject Cholera toxin en_US
dc.subject Vibrio cholerae en_US
dc.title Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae en_US
dc.type Article en_US
dc.identifier.apacitation Ssemakalu, C., Le Roux, W. J., & Pillay, M. (2013). Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae. http://hdl.handle.net/10204/7259 en_ZA
dc.identifier.chicagocitation Ssemakalu, CC, Wouter J Le Roux, and M Pillay "Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae." (2013) http://hdl.handle.net/10204/7259 en_ZA
dc.identifier.vancouvercitation Ssemakalu C, Le Roux WJ, Pillay M. Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae. 2013; http://hdl.handle.net/10204/7259. en_ZA
dc.identifier.ris TY - Article AU - Ssemakalu, CC AU - Le Roux, Wouter J AU - Pillay, M AB - Cholera toxin is the aetiological agent of cholera – a deadly waterborne disease acquired through the consumption of untreated water contaminated with CTX bacteriophage harbouring strains of V. cholerae. Solar disinfection is a re-emerging technique that relies on the ultraviolet component of sunlight to inactivate the growth of Vibrio cholerae in water, rendering the water microbiologically safe for consumption. However, studies have shown that DNA damaging agents, such as ultraviolet light, induce the replication of the CTX bacteriophage with subsequent expression of the cholera toxin. In this study we investigated the impact of solar irradiation on the secretion of cholera toxin by toxigenic strains of V. cholerae in water. The cholera toxin ELISA assay, qualitative and quantitative real-time PCR as well as growth on solid media were used to determine cholera toxin secretion, DNA integrity and growth of the bacteria after 7 h and 31 h of solar irradiation. Solar irradiation in water reduced the integrity of DNA, inactivated the growth of V. cholerae and, most importantly, prevented the secretion of detectable levels of cholera toxin. This finding is encouraging for resource-poor communities that may rely on solar disinfection to alleviate the burden of cholera-related fatalities. DA - 2013-09 DB - ResearchSpace DP - CSIR KW - Solar disinfection KW - SODIS KW - Cholera KW - Cholera toxin KW - Vibrio cholerae LK - https://researchspace.csir.co.za PY - 2013 SM - 0038-2353 T1 - Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae TI - Impact of solar irradiation on cholera toxin secretion by different strains of Vibrio cholerae UR - http://hdl.handle.net/10204/7259 ER - en_ZA


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