dc.contributor.author |
Sanyika, TW
|
|
dc.contributor.author |
Stafford, William HL
|
|
dc.contributor.author |
Cowan, DA
|
|
dc.date.accessioned |
2013-04-18T10:46:53Z |
|
dc.date.available |
2013-04-18T10:46:53Z |
|
dc.date.issued |
2012-08 |
|
dc.identifier.citation |
Sanyika, T.W, Stafford, W, Cowan, D.A. 2012. The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. Polar Biology, vol. 35(8), pp 1129-1141 |
en_US |
dc.identifier.issn |
0722-4060 |
|
dc.identifier.uri |
http://link.springer.com/content/pdf/10.1007/s00300-012-1160-0
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/6697
|
|
dc.description |
Copyright: 2012 Springer Verlag. This is an ABSTRACT ONLY. The definitive version is published in Polar Biology, vol. 35(8), pp 1129-1141 |
en_US |
dc.description.abstract |
Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microbial diversity and ecological determinants in this harsh Sub-Antarctic environment are largely uncharacterized. Actinobacteria have diverse ecological functions related to soil and plant functioning. This study was aimed at characterizing the diversity and community structures of the dominant actinobacteria in the distinct habitats and to identify their determinant soil and plant characteristics. Using the 16S rRNA gene, the denaturing gradient gel electrophoresis patterns and clone library diversity were correlated with the soil and plant characteristics. Multivariate statistical methods were also used to identify determinant soil and plant characteristics. Salinity and pH were the most important soil determinants, and a number of important site-specific plant species may have been important. The Coastal Fellfield Habitat was dominated by sequences of the suborders Micrococcineae (44%) and Propionibacterineae (18%), with salinity identified as the principal determinant. The Cotula Herbfield Habitat was dominated by Frankineae (37%) and Streptosporangineae (38%), which were correlated with organic nutrient concentrations. The Wet Mire Habitat was dominated by Acidimicrobineae (61%), with moisture and organic carbon content as principal components. Culture-dependent studies were complementary to culture-independent studies with the majority of actinobacteria isolated not identified in 16S rRNA gene clone libraries. This study demonstrates how the soil physiochemical characteristics and plant species independently determine the community structures of the dominant actinobacteria in distinct ecological habitats. These factors subsequently influence their ecological adaptation, roles and functions. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Springer Verlag |
en_US |
dc.relation.ispartofseries |
Workflow;9377 |
|
dc.subject |
Actinobacteria |
en_US |
dc.subject |
Marion Island terrestrial habitats |
en_US |
dc.subject |
Soil determinants |
en_US |
dc.subject |
Plant determinants |
en_US |
dc.subject |
Microbial diversity |
en_US |
dc.subject |
Sub-Antarctic environment |
en_US |
dc.title |
The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Sanyika, T., Stafford, W. H., & Cowan, D. (2012). The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. http://hdl.handle.net/10204/6697 |
en_ZA |
dc.identifier.chicagocitation |
Sanyika, TW, William HL Stafford, and DA Cowan "The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica." (2012) http://hdl.handle.net/10204/6697 |
en_ZA |
dc.identifier.vancouvercitation |
Sanyika T, Stafford WH, Cowan D. The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. 2012; http://hdl.handle.net/10204/6697. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Sanyika, TW
AU - Stafford, William HL
AU - Cowan, DA
AB - Marion Island is a Sub-Antarctic island made up of distinct ecological habitats based on soil physiochemical, plant cover and physical characteristics. The microbial diversity and ecological determinants in this harsh Sub-Antarctic environment are largely uncharacterized. Actinobacteria have diverse ecological functions related to soil and plant functioning. This study was aimed at characterizing the diversity and community structures of the dominant actinobacteria in the distinct habitats and to identify their determinant soil and plant characteristics. Using the 16S rRNA gene, the denaturing gradient gel electrophoresis patterns and clone library diversity were correlated with the soil and plant characteristics. Multivariate statistical methods were also used to identify determinant soil and plant characteristics. Salinity and pH were the most important soil determinants, and a number of important site-specific plant species may have been important. The Coastal Fellfield Habitat was dominated by sequences of the suborders Micrococcineae (44%) and Propionibacterineae (18%), with salinity identified as the principal determinant. The Cotula Herbfield Habitat was dominated by Frankineae (37%) and Streptosporangineae (38%), which were correlated with organic nutrient concentrations. The Wet Mire Habitat was dominated by Acidimicrobineae (61%), with moisture and organic carbon content as principal components. Culture-dependent studies were complementary to culture-independent studies with the majority of actinobacteria isolated not identified in 16S rRNA gene clone libraries. This study demonstrates how the soil physiochemical characteristics and plant species independently determine the community structures of the dominant actinobacteria in distinct ecological habitats. These factors subsequently influence their ecological adaptation, roles and functions.
DA - 2012-08
DB - ResearchSpace
DP - CSIR
KW - Actinobacteria
KW - Marion Island terrestrial habitats
KW - Soil determinants
KW - Plant determinants
KW - Microbial diversity
KW - Sub-Antarctic environment
LK - https://researchspace.csir.co.za
PY - 2012
SM - 0722-4060
T1 - The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica
TI - The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica
UR - http://hdl.handle.net/10204/6697
ER -
|
en_ZA |