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Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions

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dc.contributor.author Madala, NE
dc.contributor.author Steenkamp, PA
dc.contributor.author Piater, LA
dc.contributor.author Dubery, IA
dc.date.accessioned 2013-04-17T10:13:21Z
dc.date.available 2013-04-17T10:13:21Z
dc.date.issued 2012-07
dc.identifier.citation Madala, N.E, Steenkamp, P.A, Piater, L.A and Dubery, I.A. 2012. Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. Biotechnology Letters, vol. 34(7), pp 1351-1356 en_US
dc.identifier.issn 0141-5492
dc.identifier.uri http://link.springer.com/article/10.1007/s10529-012-0909-4#
dc.identifier.uri http://hdl.handle.net/10204/6671
dc.description Copyright: 2012 Springer Netherlands. This is an ABSTRACT ONLY. The definitive version is published in Biotechnology Letters, vol.34(7), pp 1351-1356 en_US
dc.description.abstract Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-methoxyisonitrosoacetophenone in 54 %yield over 18 h. Unconverted INAP was at 33 lM. UPLC–MS/MS analyses with MassFragment software were used for metabolite identification. INAP had been hydroxylated at its meta- and para-positions as well as undergoing subsequent methoxylation and glycosylation. INAP is thus recognized by the enzymatic machinery of the phenylpropanoid pathway and is converted to a molecule with a substitution pattern similar to ferulic acid. en_US
dc.language.iso en en_US
dc.publisher Springer Netherlands en_US
dc.relation.ispartofseries Workflow;10301
dc.subject Biotransformation en_US
dc.subject Isonitrosoacetophenone en_US
dc.subject 2-keto-2-phenyl-acetaldoxime en_US
dc.subject Metabolism en_US
dc.subject Nicotiana tabacum en_US
dc.subject Solanaceae en_US
dc.subject Xenobiotics en_US
dc.title Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions en_US
dc.type Article en_US
dc.identifier.apacitation Madala, N., Steenkamp, P., Piater, L., & Dubery, I. (2012). Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. http://hdl.handle.net/10204/6671 en_ZA
dc.identifier.chicagocitation Madala, NE, PA Steenkamp, LA Piater, and IA Dubery "Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions." (2012) http://hdl.handle.net/10204/6671 en_ZA
dc.identifier.vancouvercitation Madala N, Steenkamp P, Piater L, Dubery I. Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. 2012; http://hdl.handle.net/10204/6671. en_ZA
dc.identifier.ris TY - Article AU - Madala, NE AU - Steenkamp, PA AU - Piater, LA AU - Dubery, IA AB - Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-methoxyisonitrosoacetophenone in 54 %yield over 18 h. Unconverted INAP was at 33 lM. UPLC–MS/MS analyses with MassFragment software were used for metabolite identification. INAP had been hydroxylated at its meta- and para-positions as well as undergoing subsequent methoxylation and glycosylation. INAP is thus recognized by the enzymatic machinery of the phenylpropanoid pathway and is converted to a molecule with a substitution pattern similar to ferulic acid. DA - 2012-07 DB - ResearchSpace DP - CSIR KW - Biotransformation KW - Isonitrosoacetophenone KW - 2-keto-2-phenyl-acetaldoxime KW - Metabolism KW - Nicotiana tabacum KW - Solanaceae KW - Xenobiotics LK - https://researchspace.csir.co.za PY - 2012 SM - 0141-5492 T1 - Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions TI - Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions UR - http://hdl.handle.net/10204/6671 ER - en_ZA


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