dc.contributor.author |
Zawaira, A
|
|
dc.contributor.author |
Pooran, A
|
|
dc.contributor.author |
Barichievy, S
|
|
dc.contributor.author |
Chopera, D
|
|
dc.date.accessioned |
2012-03-28T09:48:09Z |
|
dc.date.available |
2012-03-28T09:48:09Z |
|
dc.date.issued |
2011-09 |
|
dc.identifier.citation |
Zawaira, A, Pooran, A, Barichievy, S and Chopera, D. 2011. A discussion of molecular biology methods for protein engineering. Molecular Biotechnology, 36pp |
en_US |
dc.identifier.issn |
1073-6085 |
|
dc.identifier.issn |
1559-0305 |
|
dc.identifier.uri |
http://www.springerlink.com/content/675581q2558p0535/
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/5690
|
|
dc.description |
Copyright: 2011 Humana Press. This is an ABSTRACT ONLY. |
en_US |
dc.description.abstract |
A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Humana Press |
en_US |
dc.relation.ispartofseries |
Workflow;8502 |
|
dc.subject |
Molecular biology |
en_US |
dc.subject |
Molecular biology methods |
en_US |
dc.subject |
Protein engineering |
en_US |
dc.subject |
Protein expression |
en_US |
dc.subject |
Protein expression methods |
en_US |
dc.subject |
Mutagenesis |
en_US |
dc.subject |
Site-directed mutagenesis |
en_US |
dc.subject |
Insertion mutagenesis |
en_US |
dc.subject |
Deletion mutagenesis |
en_US |
dc.subject |
Polymerase chain reaction |
en_US |
dc.subject |
Molecular cloning |
en_US |
dc.subject |
Recombinant protein expression |
en_US |
dc.subject |
Recombinant plasmid |
en_US |
dc.title |
A discussion of molecular biology methods for protein engineering |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Zawaira, A., Pooran, A., Barichievy, S., & Chopera, D. (2011). A discussion of molecular biology methods for protein engineering. http://hdl.handle.net/10204/5690 |
en_ZA |
dc.identifier.chicagocitation |
Zawaira, A, A Pooran, S Barichievy, and D Chopera "A discussion of molecular biology methods for protein engineering." (2011) http://hdl.handle.net/10204/5690 |
en_ZA |
dc.identifier.vancouvercitation |
Zawaira A, Pooran A, Barichievy S, Chopera D. A discussion of molecular biology methods for protein engineering. 2011; http://hdl.handle.net/10204/5690. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Zawaira, A
AU - Pooran, A
AU - Barichievy, S
AU - Chopera, D
AB - A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.
DA - 2011-09
DB - ResearchSpace
DP - CSIR
KW - Molecular biology
KW - Molecular biology methods
KW - Protein engineering
KW - Protein expression
KW - Protein expression methods
KW - Mutagenesis
KW - Site-directed mutagenesis
KW - Insertion mutagenesis
KW - Deletion mutagenesis
KW - Polymerase chain reaction
KW - Molecular cloning
KW - Recombinant protein expression
KW - Recombinant plasmid
LK - https://researchspace.csir.co.za
PY - 2011
SM - 1073-6085
SM - 1559-0305
T1 - A discussion of molecular biology methods for protein engineering
TI - A discussion of molecular biology methods for protein engineering
UR - http://hdl.handle.net/10204/5690
ER -
|
en_ZA |