dc.contributor.author |
Barros, E
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|
dc.contributor.author |
Nelson, SW
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|
dc.date.accessioned |
2011-03-28T08:52:21Z |
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dc.date.available |
2011-03-28T08:52:21Z |
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dc.date.issued |
2010-06 |
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dc.identifier.citation |
Barros, E and Nelson, SW. 2010. Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. South African Journal of Science, 106(5/6), pp 4 |
en_US |
dc.identifier.issn |
0038-2353 |
|
dc.identifier.uri |
http://www.scielo.org.za/pdf/sajs/v106n5-6/v106n5-6a18.pdf
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|
dc.identifier.uri |
http://hdl.handle.net/10204/4925
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|
dc.description |
Copyright: 2010 Academy of Science of South Africa |
en_US |
dc.description.abstract |
The aim of this study was to use conventional breeding to increase the production in maize of the human monoclonal antibody 2G12, known to have potential therapeutic properties in the prevention of HIV-1 transmission. The recombinant antibody, together with a fluorescent marker, was introduced into two South African high-performing maize elite inbred lines by crossing them with a transgenic maize line that had been transformed with the monoclonal antibody 2G12. The effect of breeding to produce high-expressing recombinant hybrid seed was evaluated by comparing 2G12 production in the different breeding lines with the original maize line. 'Good production practice standards' were followed throughout the breeding programme. 'Conventional drug regulations' adapted to plant-made pharmaceuticals were also followed, with the seeds being stored in a 'master seed bank'. The maize hybrid expressed a higher level of the antibody than the recombinant maize elite lines. This plant-derived antibody provides a means of producing a microbicide component that could be used with other HIV-neutralising antibodies as an additional approach to prevent HIV infection. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Academy of Science of South Africa |
en_US |
dc.relation.ispartofseries |
Workflow;4028 |
|
dc.subject |
HIV-1 |
en_US |
dc.subject |
Biopharming |
en_US |
dc.subject |
Microbicide |
en_US |
dc.subject |
Monoclonal antibody |
en_US |
dc.subject |
Maize hybrid |
en_US |
dc.subject |
AIDS |
en_US |
dc.subject |
2G12 |
en_US |
dc.subject |
HIV vaccine development |
en_US |
dc.subject |
HIV transmission |
en_US |
dc.subject |
high yielding recombinant maize hybrid |
en_US |
dc.title |
Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Barros, E., & Nelson, S. (2010). Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. http://hdl.handle.net/10204/4925 |
en_ZA |
dc.identifier.chicagocitation |
Barros, E, and SW Nelson "Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission." (2010) http://hdl.handle.net/10204/4925 |
en_ZA |
dc.identifier.vancouvercitation |
Barros E, Nelson S. Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. 2010; http://hdl.handle.net/10204/4925. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Barros, E
AU - Nelson, SW
AB - The aim of this study was to use conventional breeding to increase the production in maize of the human monoclonal antibody 2G12, known to have potential therapeutic properties in the prevention of HIV-1 transmission. The recombinant antibody, together with a fluorescent marker, was introduced into two South African high-performing maize elite inbred lines by crossing them with a transgenic maize line that had been transformed with the monoclonal antibody 2G12. The effect of breeding to produce high-expressing recombinant hybrid seed was evaluated by comparing 2G12 production in the different breeding lines with the original maize line. 'Good production practice standards' were followed throughout the breeding programme. 'Conventional drug regulations' adapted to plant-made pharmaceuticals were also followed, with the seeds being stored in a 'master seed bank'. The maize hybrid expressed a higher level of the antibody than the recombinant maize elite lines. This plant-derived antibody provides a means of producing a microbicide component that could be used with other HIV-neutralising antibodies as an additional approach to prevent HIV infection.
DA - 2010-06
DB - ResearchSpace
DP - CSIR
KW - HIV-1
KW - Biopharming
KW - Microbicide
KW - Monoclonal antibody
KW - Maize hybrid
KW - AIDS
KW - 2G12
KW - HIV vaccine development
KW - HIV transmission
KW - high yielding recombinant maize hybrid
LK - https://researchspace.csir.co.za
PY - 2010
SM - 0038-2353
T1 - Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission
TI - Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission
UR - http://hdl.handle.net/10204/4925
ER -
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en_ZA |