dc.contributor.author |
Lalloo, Rajesh
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dc.contributor.author |
Maharajh, Dheepak M
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|
dc.contributor.author |
Gorgens, J
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dc.contributor.author |
Gardiner, NS
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dc.contributor.author |
Gorgens, JF
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dc.date.accessioned |
2010-07-12T13:49:03Z |
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dc.date.available |
2010-07-12T13:49:03Z |
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dc.date.issued |
2009-05 |
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dc.identifier.citation |
Lallo, R, Maharajh, D,Gorgens, J, et al. 2009. High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation. Applied Microbiology and Biotechnology, Vol 83(1), pp 59-66 |
en |
dc.identifier.issn |
0175-7598 |
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dc.identifier.uri |
http://hdl.handle.net/10204/4060
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dc.description |
Copyright:2009 Springer Berlin. This is a post print version. The definitive version was published in the Journal of Applied Microbiology and Biotechnology, Vol 83(1), pp 59-66 |
en |
dc.description.abstract |
Previous studies have demonstrated the efficacy of our Bacillus cereus isolate (NRRL 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. In vivo efficacy and tolerance to a range of physiological conditions in systems used to rear Cyprinus carpio make this isolate an excellent candidate for aquaculture applications. Production cost is an important consideration in development of commercially relevant biological products, and this study examines the optimization of nutrient supplementation, which has an impact on high-density production of spores by fermentation. Corn steep liquor (CSL) was identified as a lower cost and more effective nutrient source in comparison to conventional nutrient substrates, in particular yeast extract and nutrient broth. The improved sporulation performance of B. cereus could be related to the increased availability of free amino acids, carbohydrates, and minerals in CSL, which had a positive effect on sporulation efficiency. The impact of nutrient concentration on spore yield and productivity was modeled to develop a tool for optimization of nutrient concentration in fermentation. An excellent fit of the model was confirmed in laboratory fermentation studies. A cost comparison revealed that production using liquid phytase and ultrafiltered-treated CSL was less expensive than spray-dried CSL and supported cultivation of B. cereus spores at densities higher than 1 × 1010 CFU ml−1 |
en |
dc.language.iso |
en |
en |
dc.publisher |
Springer Berlin |
en |
dc.subject |
Bacillus cereus |
en |
dc.subject |
Fermentation |
en |
dc.subject |
Aquaculture |
en |
dc.subject |
Biological agent |
en |
dc.subject |
Nutrient supplementaion |
en |
dc.title |
High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation |
en |
dc.type |
Article |
en |
dc.identifier.apacitation |
Lalloo, R., Maharajh, D. M., Gorgens, J., Gardiner, N., & Gorgens, J. (2009). High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation. http://hdl.handle.net/10204/4060 |
en_ZA |
dc.identifier.chicagocitation |
Lalloo, Rajesh, Dheepak M Maharajh, J Gorgens, NS Gardiner, and JF Gorgens "High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation." (2009) http://hdl.handle.net/10204/4060 |
en_ZA |
dc.identifier.vancouvercitation |
Lalloo R, Maharajh DM, Gorgens J, Gardiner N, Gorgens J. High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation. 2009; http://hdl.handle.net/10204/4060. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Lalloo, Rajesh
AU - Maharajh, Dheepak M
AU - Gorgens, J
AU - Gardiner, NS
AU - Gorgens, JF
AB - Previous studies have demonstrated the efficacy of our Bacillus cereus isolate (NRRL 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. In vivo efficacy and tolerance to a range of physiological conditions in systems used to rear Cyprinus carpio make this isolate an excellent candidate for aquaculture applications. Production cost is an important consideration in development of commercially relevant biological products, and this study examines the optimization of nutrient supplementation, which has an impact on high-density production of spores by fermentation. Corn steep liquor (CSL) was identified as a lower cost and more effective nutrient source in comparison to conventional nutrient substrates, in particular yeast extract and nutrient broth. The improved sporulation performance of B. cereus could be related to the increased availability of free amino acids, carbohydrates, and minerals in CSL, which had a positive effect on sporulation efficiency. The impact of nutrient concentration on spore yield and productivity was modeled to develop a tool for optimization of nutrient concentration in fermentation. An excellent fit of the model was confirmed in laboratory fermentation studies. A cost comparison revealed that production using liquid phytase and ultrafiltered-treated CSL was less expensive than spray-dried CSL and supported cultivation of B. cereus spores at densities higher than 1 × 1010 CFU ml−1
DA - 2009-05
DB - ResearchSpace
DP - CSIR
KW - Bacillus cereus
KW - Fermentation
KW - Aquaculture
KW - Biological agent
KW - Nutrient supplementaion
LK - https://researchspace.csir.co.za
PY - 2009
SM - 0175-7598
T1 - High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation
TI - High-density spore production of a B. cereus aquaculture biological agent by nutrient supplementation
UR - http://hdl.handle.net/10204/4060
ER -
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en_ZA |