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Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation

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dc.contributor.author Visser, Daniel F
dc.contributor.author Hennessy, F
dc.contributor.author Chhiba, Varsha P
dc.contributor.author Lakay, F
dc.contributor.author van Heerden, E
dc.contributor.author Piater, L
dc.contributor.author Moller, C
dc.contributor.author Litthauer, D
dc.contributor.author Brady, D
dc.date.accessioned 2009-01-21T12:24:46Z
dc.date.available 2009-01-21T12:24:46Z
dc.date.issued 2006-10
dc.identifier.citation Visser, DF, Hennessy, F, Chhiba, VP et al. 2006. Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. University of the Free State, pp 1 en
dc.identifier.uri http://hdl.handle.net/10204/2877
dc.description.abstract Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from four South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA isolated; this DNA was sequenced and analysed using BLAST en
dc.language.iso en en
dc.subject DNA en
dc.subject BioPAD en
dc.subject Amylase en
dc.subject Nitrilase en
dc.subject Protease en
dc.subject Lipase en
dc.title Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation en
dc.type Conference Presentation en
dc.identifier.apacitation Visser, D. F., Hennessy, F., Chhiba, V. P., Lakay, F., van Heerden, E., Piater, L., ... Brady, D. (2006). Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. http://hdl.handle.net/10204/2877 en_ZA
dc.identifier.chicagocitation Visser, Daniel F, F Hennessy, Varsha P Chhiba, F Lakay, E van Heerden, L Piater, C Moller, D Litthauer, and D Brady. "Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation." (2006): http://hdl.handle.net/10204/2877 en_ZA
dc.identifier.vancouvercitation Visser DF, Hennessy F, Chhiba VP, Lakay F, van Heerden E, Piater L, et al, Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation; 2006. http://hdl.handle.net/10204/2877 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Visser, Daniel F AU - Hennessy, F AU - Chhiba, Varsha P AU - Lakay, F AU - van Heerden, E AU - Piater, L AU - Moller, C AU - Litthauer, D AU - Brady, D AB - Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from four South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA isolated; this DNA was sequenced and analysed using BLAST DA - 2006-10 DB - ResearchSpace DP - CSIR KW - DNA KW - BioPAD KW - Amylase KW - Nitrilase KW - Protease KW - Lipase LK - https://researchspace.csir.co.za PY - 2006 T1 - Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation TI - Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation UR - http://hdl.handle.net/10204/2877 ER - en_ZA


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