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Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica

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dc.contributor.author Kahari, D
dc.contributor.author Koorsen, G
dc.contributor.author Stoychev, Stoyan H
dc.contributor.author Chipeta, Z
dc.contributor.author Labuschagne, M
dc.contributor.author Van Zyl, P
dc.contributor.author Nthangeni, B
dc.date.accessioned 2009-01-21T08:50:07Z
dc.date.available 2009-01-21T08:50:07Z
dc.date.issued 2008-11
dc.identifier.citation Kahari, D, Koorsen, G, Stoychev, S et al. 2008. Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica. Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008, pp 1 en
dc.identifier.uri http://hdl.handle.net/10204/2866
dc.description Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008 en
dc.description.abstract The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating glycan profiling techniques. The gene encoding Lip2 was cloned as a C-terminally His-tagged protein, expressed in Yarrowia lipolytica (Madzak, C et al;2004) and the glycan composition of the purified protein was analysed by HPLC and MALDITOF. The HPLC techniques was also used to profile the glycan composition of EPO expressed in Yarrowia lipolytica yeast. en
dc.language.iso en en
dc.publisher CSIR en
dc.subject Protein en
dc.subject Yarrowia lipolytica yeast en
dc.subject N-linked glycans en
dc.title Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica en
dc.type Conference Presentation en
dc.identifier.apacitation Kahari, D., Koorsen, G., Stoychev, S. H., Chipeta, Z., Labuschagne, M., Van Zyl, P., & Nthangeni, B. (2008). Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica. CSIR. http://hdl.handle.net/10204/2866 en_ZA
dc.identifier.chicagocitation Kahari, D, G Koorsen, Stoyan H Stoychev, Z Chipeta, M Labuschagne, P Van Zyl, and B Nthangeni. "Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica." (2008): http://hdl.handle.net/10204/2866 en_ZA
dc.identifier.vancouvercitation Kahari D, Koorsen G, Stoychev SH, Chipeta Z, Labuschagne M, Van Zyl P, et al, Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica; CSIR; 2008. http://hdl.handle.net/10204/2866 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Kahari, D AU - Koorsen, G AU - Stoychev, Stoyan H AU - Chipeta, Z AU - Labuschagne, M AU - Van Zyl, P AU - Nthangeni, B AB - The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating glycan profiling techniques. The gene encoding Lip2 was cloned as a C-terminally His-tagged protein, expressed in Yarrowia lipolytica (Madzak, C et al;2004) and the glycan composition of the purified protein was analysed by HPLC and MALDITOF. The HPLC techniques was also used to profile the glycan composition of EPO expressed in Yarrowia lipolytica yeast. DA - 2008-11 DB - ResearchSpace DP - CSIR KW - Protein KW - Yarrowia lipolytica yeast KW - N-linked glycans LK - https://researchspace.csir.co.za PY - 2008 T1 - Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica TI - Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica UR - http://hdl.handle.net/10204/2866 ER - en_ZA


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