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Use of monoclonal-antibodies for the detection of fecal bacteria in water

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dc.contributor.author Kfir, R en_US
dc.contributor.author Du Preez, M en_US
dc.contributor.author Genthe, Bettina en_US
dc.date.accessioned 2007-04-02T08:57:23Z en_US
dc.date.accessioned 2007-06-07T10:08:07Z
dc.date.available 2007-04-02T08:57:23Z en_US
dc.date.available 2007-06-07T10:08:07Z
dc.date.issued 1993 en_US
dc.identifier.citation Kfir, R, Du Preez, M and Genthe, B. 1993. Use of monoclonal-antibodies for the detection of fecal bacteria in water. Water Science and Technology, vol. 27, 04 March, pp 257-260 en_US
dc.identifier.issn 0273-1223 en_US
dc.identifier.uri http://hdl.handle.net/10204/2180 en_US
dc.identifier.uri http://hdl.handle.net/10204/2180
dc.description.abstract Monoclonal antibodies (MAbs) against heat-killed Escherichia coli and Klebsiella oxytoca originating from wastewater effluent were raised in BALB/C mice. The fusion was highly successful and three hybridomas cloned were selected to study the affinity and specificity pattern of the MAbs. The MAbs were found to react equally well with heat-killed and live bacteria when tested against their original immunogens. The MAbs showed lower specificity towards environmentally isolated faecal bacteria than a pure bacterial strain grown in the laboratory. A commercially obtained MAb showed the same specificity pattern. en_US
dc.format.extent 254813 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en en_US
dc.publisher Pergamon-Elsevier Science Ltd en_US
dc.rights Copyright: 1993 Pergamon-Elsevier Ltd en_US
dc.subject Monoclonal antibodies en_US
dc.subject Escherichia coli en_US
dc.subject Klebsiella en_US
dc.subject Fecal coliform en_US
dc.title Use of monoclonal-antibodies for the detection of fecal bacteria in water en_US
dc.type Article en_US
dc.identifier.apacitation Kfir, R., Du Preez, M., & Genthe, B. (1993). Use of monoclonal-antibodies for the detection of fecal bacteria in water. http://hdl.handle.net/10204/2180 en_ZA
dc.identifier.chicagocitation Kfir, R, M Du Preez, and Bettina Genthe "Use of monoclonal-antibodies for the detection of fecal bacteria in water." (1993) http://hdl.handle.net/10204/2180 en_ZA
dc.identifier.vancouvercitation Kfir R, Du Preez M, Genthe B. Use of monoclonal-antibodies for the detection of fecal bacteria in water. 1993; http://hdl.handle.net/10204/2180. en_ZA
dc.identifier.ris TY - Article AU - Kfir, R AU - Du Preez, M AU - Genthe, Bettina AB - Monoclonal antibodies (MAbs) against heat-killed Escherichia coli and Klebsiella oxytoca originating from wastewater effluent were raised in BALB/C mice. The fusion was highly successful and three hybridomas cloned were selected to study the affinity and specificity pattern of the MAbs. The MAbs were found to react equally well with heat-killed and live bacteria when tested against their original immunogens. The MAbs showed lower specificity towards environmentally isolated faecal bacteria than a pure bacterial strain grown in the laboratory. A commercially obtained MAb showed the same specificity pattern. DA - 1993 DB - ResearchSpace DP - CSIR KW - Monoclonal antibodies KW - Escherichia coli KW - Klebsiella KW - Fecal coliform LK - https://researchspace.csir.co.za PY - 1993 SM - 0273-1223 T1 - Use of monoclonal-antibodies for the detection of fecal bacteria in water TI - Use of monoclonal-antibodies for the detection of fecal bacteria in water UR - http://hdl.handle.net/10204/2180 ER - en_ZA


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