dc.contributor.author |
O'Kennedy, Maretha M
|
en_US |
dc.contributor.author |
Burger, JT
|
en_US |
dc.contributor.author |
Botha, FC
|
en_US |
dc.date.accessioned |
2007-02-08T08:04:25Z |
en_US |
dc.date.accessioned |
2007-06-07T10:05:48Z |
|
dc.date.available |
2007-02-08T08:04:25Z |
en_US |
dc.date.available |
2007-06-07T10:05:48Z |
|
dc.date.issued |
2004-04 |
en_US |
dc.identifier.citation |
O'Kennedy, MM, Burger, JT and Botha, FC. Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase. Plant Cell Reports, vol. 22(9), pp 684-690 |
en_US |
dc.identifier.issn |
0721-7714 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/1664
|
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/1664
|
|
dc.description.abstract |
Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic embryos were used as target tissue for bombardment using a particle inflow gun. Different culture and selection strategies were assessed in order to obtain an optimised mannose selection protocol. Stable integration of the manA gene into the genome of pearl millet was confirmed by PCR and Southern blot analysis. Stable integration of the manA transgene into the genome of pearl millet was demonstrated in T-1 and T-2 progeny of two independent transformation events with no more than four to ten copies of the transgene. Similar to results obtained from previous studies with maize and wheat, the manA gene was shown to be a superior selectable marker gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes. |
en_US |
dc.format.extent |
229835 bytes |
en_US |
dc.format.mimetype |
application/pdf |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Springer-Verlag |
en_US |
dc.rights |
Copyright: 2004 Springer-Verlag |
en_US |
dc.subject |
Immature zygotic embryos |
en_US |
dc.subject |
Biolistics |
en_US |
dc.subject |
Pennisetum glaucum (L.) R. Br |
en_US |
dc.subject |
Pearl millet |
en_US |
dc.subject |
Transgenic maize plants |
en_US |
dc.subject |
Plant sciences |
en_US |
dc.title |
Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
O'Kennedy, M. M., Burger, J., & Botha, F. (2004). Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase. http://hdl.handle.net/10204/1664 |
en_ZA |
dc.identifier.chicagocitation |
O'Kennedy, Maretha M, JT Burger, and FC Botha "Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase." (2004) http://hdl.handle.net/10204/1664 |
en_ZA |
dc.identifier.vancouvercitation |
O'Kennedy MM, Burger J, Botha F. Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase. 2004; http://hdl.handle.net/10204/1664. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - O'Kennedy, Maretha M
AU - Burger, JT
AU - Botha, FC
AB - Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic embryos were used as target tissue for bombardment using a particle inflow gun. Different culture and selection strategies were assessed in order to obtain an optimised mannose selection protocol. Stable integration of the manA gene into the genome of pearl millet was confirmed by PCR and Southern blot analysis. Stable integration of the manA transgene into the genome of pearl millet was demonstrated in T-1 and T-2 progeny of two independent transformation events with no more than four to ten copies of the transgene. Similar to results obtained from previous studies with maize and wheat, the manA gene was shown to be a superior selectable marker gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes.
DA - 2004-04
DB - ResearchSpace
DP - CSIR
KW - Immature zygotic embryos
KW - Biolistics
KW - Pennisetum glaucum (L.) R. Br
KW - Pearl millet
KW - Transgenic maize plants
KW - Plant sciences
LK - https://researchspace.csir.co.za
PY - 2004
SM - 0721-7714
T1 - Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase
TI - Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase
UR - http://hdl.handle.net/10204/1664
ER -
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en_ZA |