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Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench

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dc.contributor.author Oldach, KH en_US
dc.contributor.author Morgenstern, A en_US
dc.contributor.author Rother, S en_US
dc.contributor.author Girgi, M en_US
dc.contributor.author O'Kennedy, Maretha M en_US
dc.contributor.author Lorz, H en_US
dc.date.accessioned 2007-02-06T13:03:51Z en_US
dc.date.accessioned 2007-06-07T10:02:18Z
dc.date.available 2007-02-06T13:03:51Z en_US
dc.date.available 2007-06-07T10:02:18Z
dc.date.copyright en_US
dc.date.issued 2001-07 en_US
dc.identifier.citation Oldach, KH, et al. 2001. Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. Plant Cell Reports, vol. 20(5), pp 416-421 en_US
dc.identifier.issn 0721-7714 en_US
dc.identifier.uri http://hdl.handle.net/10204/1540 en_US
dc.identifier.uri http://hdl.handle.net/10204/1540
dc.description.abstract The authors report an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated 10-20 days after pollination and cultured on various L3 media. The influence of different parameters during the callus induction phase was examined with respect to the regeneration rate: (1) the concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and various cytokinins; (2) the addition of AgNO3; (3) the use of maltose or sucrose as a carbon source. Modifications in the phytohormones alone resulted in the regeneration of fertile sorghum plants at high efficiency. Significant increases in the regeneration rates of pearl millet genotypes were achieved by the combination of sucrose as a carbon source and silver nitrate as a potential ethylene inhibitor. en_US
dc.format.extent 248276 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en en_US
dc.publisher Springer-Verlag en_US
dc.rights Copyright: 2001 Springer-Verlag en_US
dc.source en_US
dc.subject Somatic embryogenesis en_US
dc.subject Sorghum bicolor Moench en_US
dc.subject Pearl millet immature embryos en_US
dc.subject Pennisetum glaucum (L.) R. Br en_US
dc.subject Plant sciences en_US
dc.title Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench en_US
dc.type Article en_US
dc.identifier.apacitation Oldach, K., Morgenstern, A., Rother, S., Girgi, M., O'Kennedy, M. M., & Lorz, H. (2001). Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. http://hdl.handle.net/10204/1540 en_ZA
dc.identifier.chicagocitation Oldach, KH, A Morgenstern, S Rother, M Girgi, Maretha M O'Kennedy, and H Lorz "Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench." (2001) http://hdl.handle.net/10204/1540 en_ZA
dc.identifier.vancouvercitation Oldach K, Morgenstern A, Rother S, Girgi M, O'Kennedy MM, Lorz H. Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. 2001; http://hdl.handle.net/10204/1540. en_ZA
dc.identifier.ris TY - Article AU - Oldach, KH AU - Morgenstern, A AU - Rother, S AU - Girgi, M AU - O'Kennedy, Maretha M AU - Lorz, H AB - The authors report an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated 10-20 days after pollination and cultured on various L3 media. The influence of different parameters during the callus induction phase was examined with respect to the regeneration rate: (1) the concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and various cytokinins; (2) the addition of AgNO3; (3) the use of maltose or sucrose as a carbon source. Modifications in the phytohormones alone resulted in the regeneration of fertile sorghum plants at high efficiency. Significant increases in the regeneration rates of pearl millet genotypes were achieved by the combination of sucrose as a carbon source and silver nitrate as a potential ethylene inhibitor. DA - 2001-07 DB - ResearchSpace DP - CSIR KW - Somatic embryogenesis KW - Sorghum bicolor Moench KW - Pearl millet immature embryos KW - Pennisetum glaucum (L.) R. Br KW - Plant sciences LK - https://researchspace.csir.co.za PY - 2001 SM - 0721-7714 T1 - Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench TI - Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench UR - http://hdl.handle.net/10204/1540 ER - en_ZA


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