dc.contributor.author |
Steenkamp, Lucia H
|
en_US |
dc.contributor.author |
Brady, D
|
en_US |
dc.date.accessioned |
2007-02-06T09:08:53Z |
en_US |
dc.date.accessioned |
2007-06-07T10:06:34Z |
|
dc.date.available |
2007-02-06T09:08:53Z |
en_US |
dc.date.available |
2007-06-07T10:06:34Z |
|
dc.date.copyright |
|
en_US |
dc.date.issued |
2003-03-03 |
en_US |
dc.identifier.citation |
Steenkamp, L.H. and Brady, D. Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. Enzyme and Microbial Technology, vol. 32, 04 March, pp 472-477 |
en_US |
dc.identifier.issn |
0141-0229 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/1495
|
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/1495
|
|
dc.description.abstract |
This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the conversion will reduce the mass of material that requires racemisation and recycling. Hence the aim was to find an enzyme that yields (S)-naproxen with an enantiomeric excess of more than 98%, a substrate conversion in excess of 40% of the race mate, and an E of >100. (R, S)-Naproxen ethyl ester (NEE) (50 mg) was used as substrate for enzyme hydrolysis reactions at 37 degrees for 4 h. Biocatalyst screening was performed in buffered aqueous solvent on a I ml scale. The reactions were stopped with 2 ml MeCN, filtered through cotton wool and analysed by HPLC to determine the percentage m/m and R/S ratio. Eight commercially available enzymes were selected for optimisation of enantioselectivity through statistically designed experiments where the reaction conditions were varied. ChiroCLEC-CR from Altus and ESL001-01 from Diversa provided acceptable enantiomeric excess, but only ChiroCLEC-CR met the specification set for the enantiomeric ratio (E). |
en_US |
dc.format.extent |
84125 bytes |
en_US |
dc.format.mimetype |
application/pdf |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier Science Inc |
en_US |
dc.rights |
Copyright: 2003 Elsevier Science Inc |
en_US |
dc.source |
|
en_US |
dc.subject |
Enantioselectivity |
en_US |
dc.subject |
Enzyme hydrolyses reactions |
en_US |
dc.subject |
Naproxen |
en_US |
dc.subject |
Enzymes |
en_US |
dc.subject |
Esterase |
en_US |
dc.subject |
Lipase |
en_US |
dc.title |
Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Steenkamp, L. H., & Brady, D. (2003). Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. http://hdl.handle.net/10204/1495 |
en_ZA |
dc.identifier.chicagocitation |
Steenkamp, Lucia H, and D Brady "Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester." (2003) http://hdl.handle.net/10204/1495 |
en_ZA |
dc.identifier.vancouvercitation |
Steenkamp LH, Brady D. Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester. 2003; http://hdl.handle.net/10204/1495. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Steenkamp, Lucia H
AU - Brady, D
AB - This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the conversion will reduce the mass of material that requires racemisation and recycling. Hence the aim was to find an enzyme that yields (S)-naproxen with an enantiomeric excess of more than 98%, a substrate conversion in excess of 40% of the race mate, and an E of >100. (R, S)-Naproxen ethyl ester (NEE) (50 mg) was used as substrate for enzyme hydrolysis reactions at 37 degrees for 4 h. Biocatalyst screening was performed in buffered aqueous solvent on a I ml scale. The reactions were stopped with 2 ml MeCN, filtered through cotton wool and analysed by HPLC to determine the percentage m/m and R/S ratio. Eight commercially available enzymes were selected for optimisation of enantioselectivity through statistically designed experiments where the reaction conditions were varied. ChiroCLEC-CR from Altus and ESL001-01 from Diversa provided acceptable enantiomeric excess, but only ChiroCLEC-CR met the specification set for the enantiomeric ratio (E).
DA - 2003-03-03
DB - ResearchSpace
DP - CSIR
KW - Enantioselectivity
KW - Enzyme hydrolyses reactions
KW - Naproxen
KW - Enzymes
KW - Esterase
KW - Lipase
LK - https://researchspace.csir.co.za
PY - 2003
SM - 0141-0229
T1 - Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester
TI - Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester
UR - http://hdl.handle.net/10204/1495
ER -
|
en_ZA |