dc.contributor.author |
Hamese, Saltiel
|
|
dc.contributor.author |
Mugwanda, Kanganwiro
|
|
dc.contributor.author |
Takundwa, Mutsa M
|
|
dc.contributor.author |
Prinsloo, E
|
|
dc.contributor.author |
Thimiri Govindaraj, Deepak B
|
|
dc.date.accessioned |
2024-01-11T09:58:02Z |
|
dc.date.available |
2024-01-11T09:58:02Z |
|
dc.date.issued |
2023-12 |
|
dc.identifier.citation |
Hamese, S., Mugwanda, K., Takundwa, M.M., Prinsloo, E. & Thimiri Govindaraj, D.B. 2023. Recent advances in genome annotation and synthetic biology for the development of microbial chassis. <i>Journal of Genetic Engineering and Biotechnology, 21(156).</i> http://hdl.handle.net/10204/13505 |
en_ZA |
dc.identifier.issn |
1687-157X |
|
dc.identifier.issn |
2090-5920 |
|
dc.identifier.uri |
https://doi.org/10.1186/s43141-023-00598-3
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/13505
|
|
dc.description.abstract |
This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://jgeb.springeropen.com/articles/10.1186/s43141-023-00598-3 |
en_US |
dc.source |
Journal of Genetic Engineering and Biotechnology, 21(156) |
en_US |
dc.subject |
Synthetic biology |
en_US |
dc.subject |
Genetic engineering |
en_US |
dc.subject |
Genome annotation |
en_US |
dc.subject |
Metabolic modeling |
en_US |
dc.title |
Recent advances in genome annotation and synthetic biology for the development of microbial chassis |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
9 |
en_US |
dc.description.note |
© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. |
en_US |
dc.description.cluster |
Next Generation Health |
en_US |
dc.description.impactarea |
Synthetic Nanobiotech Biomachs |
en_US |
dc.identifier.apacitation |
Hamese, S., Mugwanda, K., Takundwa, M. M., Prinsloo, E., & Thimiri Govindaraj, D. B. (2023). Recent advances in genome annotation and synthetic biology for the development of microbial chassis. <i>Journal of Genetic Engineering and Biotechnology, 21(156)</i>, http://hdl.handle.net/10204/13505 |
en_ZA |
dc.identifier.chicagocitation |
Hamese, Saltiel, Kanganwiro Mugwanda, Mutsa M Takundwa, E Prinsloo, and Deepak B Thimiri Govindaraj "Recent advances in genome annotation and synthetic biology for the development of microbial chassis." <i>Journal of Genetic Engineering and Biotechnology, 21(156)</i> (2023) http://hdl.handle.net/10204/13505 |
en_ZA |
dc.identifier.vancouvercitation |
Hamese S, Mugwanda K, Takundwa MM, Prinsloo E, Thimiri Govindaraj DB. Recent advances in genome annotation and synthetic biology for the development of microbial chassis. Journal of Genetic Engineering and Biotechnology, 21(156). 2023; http://hdl.handle.net/10204/13505. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Hamese, Saltiel
AU - Mugwanda, Kanganwiro
AU - Takundwa, Mutsa M
AU - Prinsloo, E
AU - Thimiri Govindaraj, Deepak B
AB - This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes.
DA - 2023-12
DB - ResearchSpace
DP - CSIR
J1 - Journal of Genetic Engineering and Biotechnology, 21(156)
KW - Synthetic biology
KW - Genetic engineering
KW - Genome annotation
KW - Metabolic modeling
LK - https://researchspace.csir.co.za
PY - 2023
SM - 1687-157X
SM - 2090-5920
T1 - Recent advances in genome annotation and synthetic biology for the development of microbial chassis
TI - Recent advances in genome annotation and synthetic biology for the development of microbial chassis
UR - http://hdl.handle.net/10204/13505
ER -
|
en_ZA |
dc.identifier.worklist |
27420 |
en_US |