dc.contributor.author |
Baichan, P
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dc.contributor.author |
Naicker, Previn
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dc.contributor.author |
Augustine, TN
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dc.contributor.author |
Smith, M
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dc.contributor.author |
Candy, G
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dc.contributor.author |
Devar, J
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dc.contributor.author |
Nweke, EE
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dc.date.accessioned |
2023-05-08T06:08:11Z |
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dc.date.available |
2023-05-08T06:08:11Z |
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dc.date.issued |
2023-03 |
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dc.identifier.citation |
Baichan, P., Naicker, P., Augustine, T., Smith, M., Candy, G., Devar, J. & Nweke, E. 2023. Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry. <i>Clinical Proteomics, 20(8).</i> http://hdl.handle.net/10204/12763 |
en_ZA |
dc.identifier.issn |
1542-6416 |
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dc.identifier.issn |
1559-0275 |
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dc.identifier.uri |
https://doi.org/10.1186/s12014-023-09399-9
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dc.identifier.uri |
http://hdl.handle.net/10204/12763
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dc.description.abstract |
Background Gallbladder cancer (GBC) is a lethal cancer with a poor prognosis. The lack of specifc and sensitive biomarkers results in delayed diagnosis with most patients presenting at late stages of the disease. Furthermore, there is little known about the molecular mechanisms associated with GBC, especially in patients of African ancestry. This study aimed to determine dysregulated proteins in South African GBC patients to identify potential mechanisms of the disease progression and plausible biomarkers. Methods Tissues (27 GBC, 13 Gallstone disease, and 5 normal tissues) and blood plasma (54 GBC and 73 Benign biliary pathology) were obtained from consenting patients. Protein extraction was performed on all tissues and liquid chromatography-mass spectrometry was used for proteomic profling. A project-specifc spectral library was built using the Pulsar search algorithm. Principal component and Spearman’s rank correlation analyses were performed using PAST (V4.07b). Pathway and Network analyses were conducted using REACTOME (v3.7) and stringAPP (v1.7.0), respectively. Results In the tissue sample group, there were 62 and 194 dysregulated proteins in GBC compared to normal and gallstone groups, respectively. In the plasma group, there were 33 altered proteins in GBC compared to the benign biliary pathology group. We found 9 proteins (APOA1, APOA2, RET4, TTR, HEMO, HBB, HBA, PIGR, and APOE) to be commonly dysregulated in both tissue and plasma. Furthermore, a subset analysis demonstrated that 2 proteins, S100A8 and S100A9, were downregulated in GBC patients with GD history compared to those without. Pathway analysis showed that the dysregulated proteins in GBC patients were enriched in pathways involved in smooth muscle contraction, metabolism, ECM organization, and integrin cell surface interactions. Conclusion The identifed dysregulated proteins help in understanding GBC molecular mechanisms in our patient group. Furthermore, the alteration of specifc proteins in both tissue and plasma samples suggests their potential utility as biomarkers of GBC in this sample cohort. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-023-09399-9 |
en_US |
dc.source |
Clinical Proteomics, 20(8) |
en_US |
dc.subject |
Biomarkers |
en_US |
dc.subject |
Proteomic analysis |
en_US |
dc.subject |
Gallstone disease |
en_US |
dc.subject |
Molecular pathways |
en_US |
dc.subject |
SWATH-MS |
en_US |
dc.subject |
Proteins |
en_US |
dc.title |
Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
14 |
en_US |
dc.description.note |
© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. |
en_US |
dc.description.cluster |
Next Generation Health |
en_US |
dc.description.impactarea |
Human Molecular Diagnostics |
en_US |
dc.identifier.apacitation |
Baichan, P., Naicker, P., Augustine, T., Smith, M., Candy, G., Devar, J., & Nweke, E. (2023). Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry. <i>Clinical Proteomics, 20(8)</i>, http://hdl.handle.net/10204/12763 |
en_ZA |
dc.identifier.chicagocitation |
Baichan, P, Previn Naicker, TN Augustine, M Smith, G Candy, J Devar, and EE Nweke "Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry." <i>Clinical Proteomics, 20(8)</i> (2023) http://hdl.handle.net/10204/12763 |
en_ZA |
dc.identifier.vancouvercitation |
Baichan P, Naicker P, Augustine T, Smith M, Candy G, Devar J, et al. Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry. Clinical Proteomics, 20(8). 2023; http://hdl.handle.net/10204/12763. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Baichan, P
AU - Naicker, Previn
AU - Augustine, TN
AU - Smith, M
AU - Candy, G
AU - Devar, J
AU - Nweke, EE
AB - Background Gallbladder cancer (GBC) is a lethal cancer with a poor prognosis. The lack of specifc and sensitive biomarkers results in delayed diagnosis with most patients presenting at late stages of the disease. Furthermore, there is little known about the molecular mechanisms associated with GBC, especially in patients of African ancestry. This study aimed to determine dysregulated proteins in South African GBC patients to identify potential mechanisms of the disease progression and plausible biomarkers. Methods Tissues (27 GBC, 13 Gallstone disease, and 5 normal tissues) and blood plasma (54 GBC and 73 Benign biliary pathology) were obtained from consenting patients. Protein extraction was performed on all tissues and liquid chromatography-mass spectrometry was used for proteomic profling. A project-specifc spectral library was built using the Pulsar search algorithm. Principal component and Spearman’s rank correlation analyses were performed using PAST (V4.07b). Pathway and Network analyses were conducted using REACTOME (v3.7) and stringAPP (v1.7.0), respectively. Results In the tissue sample group, there were 62 and 194 dysregulated proteins in GBC compared to normal and gallstone groups, respectively. In the plasma group, there were 33 altered proteins in GBC compared to the benign biliary pathology group. We found 9 proteins (APOA1, APOA2, RET4, TTR, HEMO, HBB, HBA, PIGR, and APOE) to be commonly dysregulated in both tissue and plasma. Furthermore, a subset analysis demonstrated that 2 proteins, S100A8 and S100A9, were downregulated in GBC patients with GD history compared to those without. Pathway analysis showed that the dysregulated proteins in GBC patients were enriched in pathways involved in smooth muscle contraction, metabolism, ECM organization, and integrin cell surface interactions. Conclusion The identifed dysregulated proteins help in understanding GBC molecular mechanisms in our patient group. Furthermore, the alteration of specifc proteins in both tissue and plasma samples suggests their potential utility as biomarkers of GBC in this sample cohort.
DA - 2023-03
DB - ResearchSpace
DP - CSIR
J1 - Clinical Proteomics, 20(8)
KW - Biomarkers
KW - Proteomic analysis
KW - Gallstone disease
KW - Molecular pathways
KW - SWATH-MS
KW - Proteins
LK - https://researchspace.csir.co.za
PY - 2023
SM - 1542-6416
SM - 1559-0275
T1 - Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry
TI - Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry
UR - http://hdl.handle.net/10204/12763
ER -
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en_ZA |
dc.identifier.worklist |
26643 |
en_US |
dc.identifier.worklist |
Gallbladder cancer |
en_US |