dc.contributor.author |
Smith, Tanya
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dc.contributor.author |
O’Kennedy, Martha M
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|
dc.contributor.author |
Ross, CS
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dc.contributor.author |
Lewis, NS
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dc.contributor.author |
Abolnik, C
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dc.date.accessioned |
2023-03-15T10:48:53Z |
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dc.date.available |
2023-03-15T10:48:53Z |
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dc.date.issued |
2023-02 |
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dc.identifier.citation |
Smith, T., O’Kennedy, Martha M, Ross, C., Lewis, N. & Abolnik, C. 2023. The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. <i>Frontiers in Plant Science, 14.</i> http://hdl.handle.net/10204/12669 |
en_ZA |
dc.identifier.issn |
1664-462X |
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dc.identifier.uri |
https://doi.org/10.3389/fpls.2023.1130910
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dc.identifier.uri |
http://hdl.handle.net/10204/12669
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dc.description.abstract |
Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://www.frontiersin.org/articles/10.3389/fpls.2023.1130910/full |
en_US |
dc.source |
Frontiers in Plant Science, 14 |
en_US |
dc.subject |
Newcastle disease |
en_US |
dc.subject |
Virus-like particles |
en_US |
dc.subject |
VLP |
en_US |
dc.subject |
Avian disease |
en_US |
dc.subject |
Vaccines |
en_US |
dc.title |
The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
13 |
en_US |
dc.description.note |
© 2023 Smith, O’Kennedy, Ross, Lewis and Abolnik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
en_US |
dc.description.cluster |
Next Generation Health |
en_US |
dc.description.impactarea |
Vetnry Mol Diagnostics and Vac |
en_US |
dc.identifier.apacitation |
Smith, T., O’Kennedy, Martha M, Ross, C., Lewis, N., & Abolnik, C. (2023). The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. <i>Frontiers in Plant Science, 14</i>, http://hdl.handle.net/10204/12669 |
en_ZA |
dc.identifier.chicagocitation |
Smith, Tanya, O’Kennedy, Martha M, CS Ross, NS Lewis, and C Abolnik "The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines." <i>Frontiers in Plant Science, 14</i> (2023) http://hdl.handle.net/10204/12669 |
en_ZA |
dc.identifier.vancouvercitation |
Smith T, O’Kennedy, Martha M, Ross C, Lewis N, Abolnik C. The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines. Frontiers in Plant Science, 14. 2023; http://hdl.handle.net/10204/12669. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Smith, Tanya
AU - O’Kennedy, Martha M
AU - Ross, CS
AU - Lewis, NS
AU - Abolnik, C
AB - Newcastle disease (ND) is a highly contagious viral respiratory and neurological disease that has a severe impact on poultry production worldwide. In the present study, an expression platform was established for the transient production in N.bethamiana of ND virus-like particles (VLPs) for use as vaccines against ND. The expression of the ND Fusion (F) and/or Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in planta as visualized under the transmission electron microscope, and HN-containing VLPs agglutinated chicken erythrocytes with hemagglutination (HA) titres of up to 13 log2.The immunogenicity of the partially-purified ND VLPs was confirmed in specific-pathogen-free White leghorn chickens. Birds receiving a single intramuscular immunization with 1024 HA units (10 log2) of the F/HN ND VLPs administered with 20% [v/v] Emulsigen®-P adjuvant, seroconverted after 14 days with F- and HN-specific antibodies at ELISA titres of 5705.17 and HI geometric mean titres (GMTs) of 6.2 log2, respectively. Furthermore, these ND-specific antibodies successfully inhibited viral replication in vitro of two antigenically closely-related ND virus isolates, with virus-neutralization test GMTs of 3.47 and 3.4, respectively. Plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species that are highly immunogenic, cost-effective, and facilitate prompt updating to ensure improved protection against emerging ND field viruses.
DA - 2023-02
DB - ResearchSpace
DP - CSIR
J1 - Frontiers in Plant Science, 14
KW - Newcastle disease
KW - Virus-like particles
KW - VLP
KW - Avian disease
KW - Vaccines
LK - https://researchspace.csir.co.za
PY - 2023
SM - 1664-462X
T1 - The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines
TI - The production of Newcastle disease virus-like particles in Nicotiana benthamiana as potential vaccines
UR - http://hdl.handle.net/10204/12669
ER -
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en_ZA |
dc.identifier.worklist |
26564 |
en_US |