dc.contributor.author |
Abolnik, C
|
|
dc.contributor.author |
Smith, Tanya
|
|
dc.contributor.author |
Wandrag, BDR
|
|
dc.contributor.author |
Murphy, M
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|
dc.contributor.author |
Rautenbach, M
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|
dc.contributor.author |
Olibile, O
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|
dc.contributor.author |
O'Kennedy, Maretha M
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|
dc.date.accessioned |
2022-10-17T07:32:07Z |
|
dc.date.available |
2022-10-17T07:32:07Z |
|
dc.date.issued |
2022-06 |
|
dc.identifier.citation |
Abolnik, C., Smith, T., Wandrag, B., Murphy, M., Rautenbach, M., Olibile, O. & O'Kennedy, M.M. 2022. Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens. <i>Heliyon, 8(6).</i> http://hdl.handle.net/10204/12504 |
en_ZA |
dc.identifier.issn |
2405-8440 |
|
dc.identifier.uri |
Doi: 10.1016/j.heliyon.2022.e09804
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/12504
|
|
dc.description.abstract |
Avian influenza poses one of the largest known threats to global poultry production and human health, but effective poultry vaccines can reduce infections rates, production losses and prevent mortalities, and reduce viral shed to limit further disease spread. The antigenic match between a vaccine and the circulating field influenza A viruses (IAV) is a critical determinant of vaccine efficacy. Here, an Agrobacterium tumefaciens-mediated transient tobacco plant (Nicotiana benthamiana) system was used to rapidly update an H6 influenza subtype virus-like particle (VLP) vaccine expressing the hemagglutininn (HA) protein of South African H6N2 IAVs circulating in 2020. Specific pathogen free White Leghorn layer hens vaccinated twice with =125 hemagglutinating unit (HAU) doses elicited protective antibody responses associated with prevention of viral shedding, i.e. hemaglutination inhibition (HI) mean geometric titres (GMTs) of =7 log2, for at least four months before dropping to approximately 5–6 log2 for at least another two months. A single vaccination with a 250 HAU dose induced significantly higher HI GMTs compared lower or higher doses, and was thus the optimal dose for chickens. Use of an adjuvant was essential, as the plant-produced H6 HA VLP alone did not induce protective antibody responses. Plant-produced IAV VLPs enable differentiation between vaccinated and infected animals (DIVA principle), and with sucrose density gradient-purified yields of 20,000 doses per kg of plant material, this highly efficacious, safe and economical technology holds enormous potential for improving poultry health in lower and middle-income countries. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244761/ |
en_US |
dc.source |
Heliyon, 8(6) |
en_US |
dc.subject |
Avian influenza |
en_US |
dc.subject |
Chicken dose |
en_US |
dc.subject |
H6N2 subtype |
en_US |
dc.subject |
Plant-produced VLP |
en_US |
dc.title |
Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
7 |
en_US |
dc.description.note |
© 2022 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
en_US |
dc.description.cluster |
Next Generation Health |
en_US |
dc.description.impactarea |
Vetnry Mol Diagnostics and Vac |
en_US |
dc.identifier.apacitation |
Abolnik, C., Smith, T., Wandrag, B., Murphy, M., Rautenbach, M., Olibile, O., & O'Kennedy, M. M. (2022). Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens. <i>Heliyon, 8(6)</i>, http://hdl.handle.net/10204/12504 |
en_ZA |
dc.identifier.chicagocitation |
Abolnik, C, Tanya Smith, BDR Wandrag, M Murphy, M Rautenbach, O Olibile, and Martha M O'Kennedy "Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens." <i>Heliyon, 8(6)</i> (2022) http://hdl.handle.net/10204/12504 |
en_ZA |
dc.identifier.vancouvercitation |
Abolnik C, Smith T, Wandrag B, Murphy M, Rautenbach M, Olibile O, et al. Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens. Heliyon, 8(6). 2022; http://hdl.handle.net/10204/12504. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Abolnik, C
AU - Smith, Tanya
AU - Wandrag, BDR
AU - Murphy, M
AU - Rautenbach, M
AU - Olibile, O
AU - O'Kennedy, Martha M
AB - Avian influenza poses one of the largest known threats to global poultry production and human health, but effective poultry vaccines can reduce infections rates, production losses and prevent mortalities, and reduce viral shed to limit further disease spread. The antigenic match between a vaccine and the circulating field influenza A viruses (IAV) is a critical determinant of vaccine efficacy. Here, an Agrobacterium tumefaciens-mediated transient tobacco plant (Nicotiana benthamiana) system was used to rapidly update an H6 influenza subtype virus-like particle (VLP) vaccine expressing the hemagglutininn (HA) protein of South African H6N2 IAVs circulating in 2020. Specific pathogen free White Leghorn layer hens vaccinated twice with =125 hemagglutinating unit (HAU) doses elicited protective antibody responses associated with prevention of viral shedding, i.e. hemaglutination inhibition (HI) mean geometric titres (GMTs) of =7 log2, for at least four months before dropping to approximately 5–6 log2 for at least another two months. A single vaccination with a 250 HAU dose induced significantly higher HI GMTs compared lower or higher doses, and was thus the optimal dose for chickens. Use of an adjuvant was essential, as the plant-produced H6 HA VLP alone did not induce protective antibody responses. Plant-produced IAV VLPs enable differentiation between vaccinated and infected animals (DIVA principle), and with sucrose density gradient-purified yields of 20,000 doses per kg of plant material, this highly efficacious, safe and economical technology holds enormous potential for improving poultry health in lower and middle-income countries.
DA - 2022-06
DB - ResearchSpace
DP - CSIR
J1 - Heliyon, 8(6)
KW - Avian influenza
KW - Chicken dose
KW - H6N2 subtype
KW - Plant-produced VLP
LK - https://researchspace.csir.co.za
PY - 2022
SM - 2405-8440
T1 - Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens
TI - Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens
UR - http://hdl.handle.net/10204/12504
ER - |
en_ZA |
dc.identifier.worklist |
26050 |
en_US |