dc.contributor.author |
Ramdas, Veshara
|
|
dc.contributor.author |
Lalloo, Rajesh
|
|
dc.contributor.author |
Mandree, Prisha
|
|
dc.contributor.author |
Mgangira, Martin B
|
|
dc.contributor.author |
Mukaratirwa, Samson
|
|
dc.contributor.author |
Ramchuran, Santosh O
|
|
dc.date.accessioned |
2022-10-03T06:36:46Z |
|
dc.date.available |
2022-10-03T06:36:46Z |
|
dc.date.issued |
2022-02 |
|
dc.identifier.citation |
Ramdas, V., Lalloo, R., Mandree, P., Mgangira, M.B., Mukaratirwa, S. & Ramchuran, S.O. 2022. Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation. <i>Applied Sciences, 12(4).</i> http://hdl.handle.net/10204/12490 |
en_ZA |
dc.identifier.issn |
2076-3417 |
|
dc.identifier.uri |
https://doi.org/10.3390/app12041774
|
|
dc.identifier.uri |
http://hdl.handle.net/10204/12490
|
|
dc.description.abstract |
Current and future research focuses on the use of renewable technologies and materials to stabilise weak soils, of varying degrees, for road construction applications. Soil stabilisation is a method of strengthening a natural soil to meet this purpose. Our interest is in the use of bio-based components, derived from microbial growth processes, that contribute to the needed desirable strength characteristics for in situ soil stabilisation. This investigation focuses on novel Bacillus-based stabilisers obtained from the vegetative and spore growth stage. In this study, eighteen bio-based components were derived from a Bacillus licheniformisfermentation and extracted into various aqueous and non-aqueous fractions for strength property assessment. The strength properties of the treated soils (i.e., dolerite and weathered granite soil) were assessed via previously developed lab-scale equipment to rapidly pre-select the best performing fractions, (i.e., compression stress, erosion, abrasion, and water absorption tests). The effect of one superior performing prototype (a) was validated at large-scale, using standard erosion and abrasion tests (i.e., whole broth at 1.8% stabiliser concentrations), and showed resistance to abrasion (3.37 ± 0.03%) (p value = 0.0001) and resistance to erosion (33.20 ± 0.15%) (p value = 0.001). The elemental composition and microstructure of the bio-stabilised soil was determined using energy dispersive X-ray spectroscopy and scanning electron microscopy, respectively. This evaluation formed part of the selection of the best performing Bacillus derived fractions and achieved a proof of concept for the next phase of product prototype development. This study demonstrated a novel bio-mediated approach to the overall criteria for evaluation and selection of candidate product prototype/s, for stabilisation of two varying soils, and for potential application in road construction works. |
en_US |
dc.format |
Fulltext |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://www.mdpi.com/2076-3417/12/4/1774 |
en_US |
dc.source |
Applied Sciences, 12(4) |
en_US |
dc.subject |
Bacillus species |
en_US |
dc.subject |
Bio-stabilisers |
en_US |
dc.subject |
Bio-polymers |
en_US |
dc.subject |
Microbial components |
en_US |
dc.subject |
Soil stabilisation |
en_US |
dc.subject |
Unpaved roads |
en_US |
dc.title |
Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
21 |
en_US |
dc.description.note |
Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/license s/by/4.0/). |
en_US |
dc.description.cluster |
Chemicals |
en_US |
dc.description.cluster |
Smart Mobility |
en_US |
dc.description.impactarea |
Biomanufacturing Technologies |
en_US |
dc.description.impactarea |
BT: Processing |
en_US |
dc.description.impactarea |
Pavement Design and Construction |
en_US |
dc.description.impactarea |
BT: Bioprocessing |
en_US |
dc.identifier.apacitation |
Ramdas, V., Lalloo, R., Mandree, P., Mgangira, M. B., Mukaratirwa, S., & Ramchuran, S. O. (2022). Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation. <i>Applied Sciences, 12(4)</i>, http://hdl.handle.net/10204/12490 |
en_ZA |
dc.identifier.chicagocitation |
Ramdas, Veshara, Rajesh Lalloo, Prisha Mandree, Martin B Mgangira, Samson Mukaratirwa, and Santosh O Ramchuran "Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation." <i>Applied Sciences, 12(4)</i> (2022) http://hdl.handle.net/10204/12490 |
en_ZA |
dc.identifier.vancouvercitation |
Ramdas V, Lalloo R, Mandree P, Mgangira MB, Mukaratirwa S, Ramchuran SO. Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation. Applied Sciences, 12(4). 2022; http://hdl.handle.net/10204/12490. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Ramdas, Veshara
AU - Lalloo, Rajesh
AU - Mandree, Prisha
AU - Mgangira, Martin B
AU - Mukaratirwa, Samson
AU - Ramchuran, Santosh O
AB - Current and future research focuses on the use of renewable technologies and materials to stabilise weak soils, of varying degrees, for road construction applications. Soil stabilisation is a method of strengthening a natural soil to meet this purpose. Our interest is in the use of bio-based components, derived from microbial growth processes, that contribute to the needed desirable strength characteristics for in situ soil stabilisation. This investigation focuses on novel Bacillus-based stabilisers obtained from the vegetative and spore growth stage. In this study, eighteen bio-based components were derived from a Bacillus licheniformisfermentation and extracted into various aqueous and non-aqueous fractions for strength property assessment. The strength properties of the treated soils (i.e., dolerite and weathered granite soil) were assessed via previously developed lab-scale equipment to rapidly pre-select the best performing fractions, (i.e., compression stress, erosion, abrasion, and water absorption tests). The effect of one superior performing prototype (a) was validated at large-scale, using standard erosion and abrasion tests (i.e., whole broth at 1.8% stabiliser concentrations), and showed resistance to abrasion (3.37 ± 0.03%) (p value = 0.0001) and resistance to erosion (33.20 ± 0.15%) (p value = 0.001). The elemental composition and microstructure of the bio-stabilised soil was determined using energy dispersive X-ray spectroscopy and scanning electron microscopy, respectively. This evaluation formed part of the selection of the best performing Bacillus derived fractions and achieved a proof of concept for the next phase of product prototype development. This study demonstrated a novel bio-mediated approach to the overall criteria for evaluation and selection of candidate product prototype/s, for stabilisation of two varying soils, and for potential application in road construction works.
DA - 2022-02
DB - ResearchSpace
DP - CSIR
J1 - Applied Sciences, 12(4)
KW - Bacillus species
KW - Bio-stabilisers
KW - Bio-polymers
KW - Microbial components
KW - Soil stabilisation
KW - Unpaved roads
LK - https://researchspace.csir.co.za
PY - 2022
SM - 2076-3417
T1 - Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation
TI - Performance evaluation of bio-based fractions derived from Bacillus spp. for potential in situ soil stabilisation
UR - http://hdl.handle.net/10204/12490
ER -
|
en_ZA |
dc.identifier.worklist |
25939 |
en_US |