dc.contributor.author |
Mohanrasu, K
|
|
dc.contributor.author |
Guru Raj Rao, R
|
|
dc.contributor.author |
Dinesh, GH
|
|
dc.contributor.author |
Zhang, K
|
|
dc.contributor.author |
Siva Prakash, G
|
|
dc.contributor.author |
Song, D
|
|
dc.contributor.author |
Muniyasamy, Sudhakar
|
|
dc.contributor.author |
Pugazhendhi, A
|
|
dc.contributor.author |
Jeyakanthan, A
|
|
dc.contributor.author |
Arun, A
|
|
dc.date.accessioned |
2021-04-05T19:36:12Z |
|
dc.date.available |
2021-04-05T19:36:12Z |
|
dc.date.issued |
2020-07 |
|
dc.identifier.citation |
Mohanrasu, K., Guru Raj Rao, R., Dinesh, G., Zhang, K., Siva Prakash, G., Song, D., Muniyasamy, S. & Pugazhendhi, A. et al. 2020. Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. <i>Fuel, 271.</i> http://hdl.handle.net/10204/11930 |
en_ZA |
dc.identifier.issn |
0016-2361 |
|
dc.identifier.issn |
1873-7153 |
|
dc.identifier.uri |
http://hdl.handle.net/10204/11930
|
|
dc.description.abstract |
Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, 1H NMR, DSC and TGA analysis, respectively. |
en_US |
dc.format |
Abstract |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.uri |
https://doi.org/10.1016/j.fuel.2020.117522 |
en_US |
dc.relation.uri |
https://www.sciencedirect.com/science/article/pii/S0016236120305172 |
en_US |
dc.source |
Fuel, 271 |
en_US |
dc.subject |
Bioreactor |
en_US |
dc.subject |
Bacillus megaterium |
en_US |
dc.subject |
Nuclear magnetic resonance |
en_US |
dc.subject |
NMR |
en_US |
dc.subject |
Polyhydroxybutyrates |
en_US |
dc.subject |
PHAs |
en_US |
dc.subject |
Thermo gravimetric analysis |
en_US |
dc.subject |
TGA |
en_US |
dc.title |
Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium |
en_US |
dc.type |
Article |
en_US |
dc.description.pages |
9pp |
en_US |
dc.description.note |
© 2020 Elsevier Ltd. All rights reserved. Due to copyright restrictions, the attached PDF file contains the abstract of the full-text item. For access to the full-text item, please consult the publisher's website: https://www.sciencedirect.com/science/article/pii/S0016236120305172 |
en_US |
dc.description.cluster |
Chemicals |
en_US |
dc.description.impactarea |
Advanced Polymer Composites |
en_US |
dc.identifier.apacitation |
Mohanrasu, K., Guru Raj Rao, R., Dinesh, G., Zhang, K., Siva Prakash, G., Song, D., ... Arun, A. (2020). Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. <i>Fuel, 271</i>, http://hdl.handle.net/10204/11930 |
en_ZA |
dc.identifier.chicagocitation |
Mohanrasu, K, R Guru Raj Rao, GH Dinesh, K Zhang, G Siva Prakash, D Song, Sudhakar Muniyasamy, A Pugazhendhi, A Jeyakanthan, and A Arun "Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium." <i>Fuel, 271</i> (2020) http://hdl.handle.net/10204/11930 |
en_ZA |
dc.identifier.vancouvercitation |
Mohanrasu K, Guru Raj Rao R, Dinesh G, Zhang K, Siva Prakash G, Song D, et al. Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. Fuel, 271. 2020; http://hdl.handle.net/10204/11930. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Mohanrasu, K
AU - Guru Raj Rao, R
AU - Dinesh, GH
AU - Zhang, K
AU - Siva Prakash, G
AU - Song, D
AU - Muniyasamy, Sudhakar
AU - Pugazhendhi, A
AU - Jeyakanthan, A
AU - Arun, A
AB - Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, 1H NMR, DSC and TGA analysis, respectively.
DA - 2020-07
DB - ResearchSpace
DP - CSIR
J1 - Fuel, 271
KW - Bioreactor
KW - Bacillus megaterium
KW - Nuclear magnetic resonance
KW - NMR
KW - Polyhydroxybutyrates
KW - PHAs
KW - Thermo gravimetric analysis
KW - TGA
LK - https://researchspace.csir.co.za
PY - 2020
SM - 0016-2361
SM - 1873-7153
T1 - Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium
TI - Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium
UR - http://hdl.handle.net/10204/11930
ER - |
en_ZA |
dc.identifier.worklist |
24119 |
en_US |