dc.contributor.author |
Thobakgale, Setumo L
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dc.contributor.author |
Ombinda-Lemboumba, Saturnin
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dc.contributor.author |
Mthunzi-Kufa, Patience
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dc.contributor.author |
Malabi, Rudzani
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dc.date.accessioned |
2020-03-19T08:08:38Z |
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dc.date.available |
2020-03-19T08:08:38Z |
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dc.date.issued |
2019-02 |
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dc.identifier.citation |
Thobakgale, S.L., Ombinda-Lemboumba, S., Mthunzi-Kufa, P. & Malabi, R. 2019. Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres. In: Proceedings of SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088416, San Francisco, California, United States, 22 February 2019 |
en_US |
dc.identifier.isbn |
9781510624108 |
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dc.identifier.isbn |
9781510624115 |
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dc.identifier.issn |
1605-7422 |
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dc.identifier.issn |
2410-9045 |
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dc.identifier.uri |
https://doi.org/10.1117/12.2509944
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|
dc.identifier.uri |
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10884/1088416/Optimizing-and-characterizing-a-home-built-Raman-spectroscopy-optical-system/10.1117/12.2509944.full
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dc.identifier.uri |
http://hdl.handle.net/10204/11351
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dc.description |
Presented in: Proceedings of SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088416, San Francisco, California, United States, 22 February 2019. Due to copyright restrictions, the attached PDF file contains the abstract of the full-text item. For access to the full-text item, please consult the publisher's website |
en_US |
dc.description.abstract |
In the recent decade, Raman spectroscopy has played a key role in photonics as a powerful method suited for detection, diagnosis and screening applications across various industrial fields. In this work, we propose a home built Raman Spectroscopy optical system optimized for polymer detection and characterization. Once fully calibrated, the intended use for the system is to analyse various macromolecules especially biomolecules in assessment of cell based diseases. This system makes use of a 527 nm excitation laser beam of 5 µs pulse duration AT 1 kHz repetition rate and an average power of 10 mW. An Andor CCD camera attached to a grated spectrometer was used for Raman spectrum acquisition and data processing was performed using the Origin software. Polystyrene microspheres (20 µm) were diluted to various concentrations and analysed using the Raman system. The results obtained reveal that all the spectra excluding the control contained Raman peaks consistent with the documented molecular vibrations of polystyrene. Furthermore, the peak intensities and peak areas showed a direct relationship with the polymer concentration in solution. Future work will include testing polymer spheres of different sizes in order to assess the spectral differentiation capabilities of the system. Much of this work will lead to the design of Raman Spectroscopy system to be used as a diagnostic tool for point-of-care detection research. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
SPIE |
en_US |
dc.relation.ispartofseries |
Workflow;22753 |
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dc.subject |
Detection |
en_US |
dc.subject |
Polystyrene |
en_US |
dc.subject |
Raman spectroscopy |
en_US |
dc.subject |
Vibrational mode |
en_US |
dc.title |
Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres |
en_US |
dc.type |
Conference Presentation |
en_US |
dc.identifier.apacitation |
Thobakgale, S. L., Ombinda-Lemboumba, S., Mthunzi-Kufa, P., & Malabi, R. (2019). Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres. SPIE. http://hdl.handle.net/10204/11351 |
en_ZA |
dc.identifier.chicagocitation |
Thobakgale, Setumo L, Saturnin Ombinda-Lemboumba, Patience Mthunzi-Kufa, and Rudzani Malabi. "Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres." (2019): http://hdl.handle.net/10204/11351 |
en_ZA |
dc.identifier.vancouvercitation |
Thobakgale SL, Ombinda-Lemboumba S, Mthunzi-Kufa P, Malabi R, Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres; SPIE; 2019. http://hdl.handle.net/10204/11351 . |
en_ZA |
dc.identifier.ris |
TY - Conference Presentation
AU - Thobakgale, Setumo L
AU - Ombinda-Lemboumba, Saturnin
AU - Mthunzi-Kufa, Patience
AU - Malabi, Rudzani
AB - In the recent decade, Raman spectroscopy has played a key role in photonics as a powerful method suited for detection, diagnosis and screening applications across various industrial fields. In this work, we propose a home built Raman Spectroscopy optical system optimized for polymer detection and characterization. Once fully calibrated, the intended use for the system is to analyse various macromolecules especially biomolecules in assessment of cell based diseases. This system makes use of a 527 nm excitation laser beam of 5 µs pulse duration AT 1 kHz repetition rate and an average power of 10 mW. An Andor CCD camera attached to a grated spectrometer was used for Raman spectrum acquisition and data processing was performed using the Origin software. Polystyrene microspheres (20 µm) were diluted to various concentrations and analysed using the Raman system. The results obtained reveal that all the spectra excluding the control contained Raman peaks consistent with the documented molecular vibrations of polystyrene. Furthermore, the peak intensities and peak areas showed a direct relationship with the polymer concentration in solution. Future work will include testing polymer spheres of different sizes in order to assess the spectral differentiation capabilities of the system. Much of this work will lead to the design of Raman Spectroscopy system to be used as a diagnostic tool for point-of-care detection research.
DA - 2019-02
DB - ResearchSpace
DP - CSIR
KW - Detection
KW - Polystyrene
KW - Raman spectroscopy
KW - Vibrational mode
LK - https://researchspace.csir.co.za
PY - 2019
SM - 9781510624108
SM - 9781510624115
SM - 1605-7422
SM - 2410-9045
T1 - Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres
TI - Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres
UR - http://hdl.handle.net/10204/11351
ER -
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en_ZA |