dc.contributor.author |
Malabi, Rudzani
|
|
dc.contributor.author |
Manoto, Sello L
|
|
dc.contributor.author |
Ombinda-Lemboumba, Saturnin
|
|
dc.contributor.author |
Maaza, Malik
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|
dc.contributor.author |
Mthunzi-Kufa, Patience
|
|
dc.date.accessioned |
2019-08-26T07:20:49Z |
|
dc.date.available |
2019-08-26T07:20:49Z |
|
dc.date.issued |
2019-05 |
|
dc.identifier.citation |
Malabi, R., Manoto, S.L., Ombinda‐Lemboumba, S., Maaza, M. & Mthunzi‐Kufa, P. 2019. Laser‐enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus‐1 infected TZMbl cells. Journal of Biophotonics, pp. 1-9. https://doi.org/10.1002/jbio.201800424 |
en_US |
dc.identifier.issn |
1864-063X |
|
dc.identifier.issn |
1864-0648 |
|
dc.identifier.uri |
https://doi.org/10.1002/jbio.201800424
|
|
dc.identifier.uri |
https://www.ncbi.nlm.nih.gov/pubmed/31140728
|
|
dc.identifier.uri |
https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201800424
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|
dc.identifier.uri |
http://hdl.handle.net/10204/11098
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|
dc.description |
Copyright: 2019 Wiley Online Library: Due to copyright restrictions, the attached PDF file only contains the abstract version of the full-text item. For access to the full-text item, please consult the publisher's website. The definitive version of the work is published Journal of Biophotonics, pp. 1-9. https://doi.org/10.1002/jbio.201800424 |
en_US |
dc.description.abstract |
The introduction of highly active antiretroviral therapy (HAART) has significantly increased life expectancy and improved management of the human immunodeficiency virus-1 (HIV-1) disease globally. This well-established treatment regime has shown to reduce viral capacity to undetectable limits when using traditional clinical assays. The establishment of viral reservoirs during the early stages of infection are the major contributors to failure of the current regimens to eradicate HIV-1 infection since the reservoirs are not affected by antiretroviral drugs (ARVs). Therefore, advanced modification of the present treatment and investigation of novel antiretroviral drug delivery system are needed. The aim of this study was to use femtosecond (fs) laser pulses to deliver ARVs into HIV-1 infected TZMbl cells. Different ARVs were translocated into TZMbl cells using fs pulsed laser (800 nm) with optimum power of 4 µW and 10 ms laser to cell exposure time. Changes in cellular processes were evaluated using cellular morphology, viability, cytotoxicity and luciferase activity assays. Cells treated with the laser in the presence of ARVs showed a significant reduction in viral infectivity, cell viability and an increase in cytotoxicity. This study demonstrated that fs laser pulses were highly effective in delivering ARVs into HIV-1 infected TZMbl cells, causing a significant reduction in HIV-1 infection. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley Online Library |
en_US |
dc.relation.ispartofseries |
Workflow;22582 |
|
dc.subject |
Antiretroviral drugs |
en_US |
dc.subject |
Femtosecond laser pulses |
en_US |
dc.subject |
HIV-1 |
en_US |
dc.subject |
Optical drug delivery |
en_US |
dc.subject |
Photo-translocation |
en_US |
dc.subject |
TZMbl cells |
en_US |
dc.title |
Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Malabi, R., Manoto, S. L., Ombinda-Lemboumba, S., Maaza, M., & Mthunzi-Kufa, P. (2019). Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells. http://hdl.handle.net/10204/11098 |
en_ZA |
dc.identifier.chicagocitation |
Malabi, Rudzani, Sello L Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, and Patience Mthunzi-Kufa "Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells." (2019) http://hdl.handle.net/10204/11098 |
en_ZA |
dc.identifier.vancouvercitation |
Malabi R, Manoto SL, Ombinda-Lemboumba S, Maaza M, Mthunzi-Kufa P. Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells. 2019; http://hdl.handle.net/10204/11098. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Malabi, Rudzani
AU - Manoto, Sello L
AU - Ombinda-Lemboumba, Saturnin
AU - Maaza, Malik
AU - Mthunzi-Kufa, Patience
AB - The introduction of highly active antiretroviral therapy (HAART) has significantly increased life expectancy and improved management of the human immunodeficiency virus-1 (HIV-1) disease globally. This well-established treatment regime has shown to reduce viral capacity to undetectable limits when using traditional clinical assays. The establishment of viral reservoirs during the early stages of infection are the major contributors to failure of the current regimens to eradicate HIV-1 infection since the reservoirs are not affected by antiretroviral drugs (ARVs). Therefore, advanced modification of the present treatment and investigation of novel antiretroviral drug delivery system are needed. The aim of this study was to use femtosecond (fs) laser pulses to deliver ARVs into HIV-1 infected TZMbl cells. Different ARVs were translocated into TZMbl cells using fs pulsed laser (800 nm) with optimum power of 4 µW and 10 ms laser to cell exposure time. Changes in cellular processes were evaluated using cellular morphology, viability, cytotoxicity and luciferase activity assays. Cells treated with the laser in the presence of ARVs showed a significant reduction in viral infectivity, cell viability and an increase in cytotoxicity. This study demonstrated that fs laser pulses were highly effective in delivering ARVs into HIV-1 infected TZMbl cells, causing a significant reduction in HIV-1 infection.
DA - 2019-05
DB - ResearchSpace
DP - CSIR
KW - Antiretroviral drugs
KW - Femtosecond laser pulses
KW - HIV-1
KW - Optical drug delivery
KW - Photo-translocation
KW - TZMbl cells
LK - https://researchspace.csir.co.za
PY - 2019
SM - 1864-063X
SM - 1864-0648
T1 - Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells
TI - Laser-enhanced drug delivery of antiretroviral drugs into human immunodeficiency virus-1 infected TZMbl cells
UR - http://hdl.handle.net/10204/11098
ER -
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en_ZA |