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Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

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dc.contributor.author Mhlongo, MI
dc.contributor.author Tugizimana, F
dc.contributor.author Piater, LA
dc.contributor.author Steenkamp, Paul A
dc.contributor.author Madala, NE
dc.contributor.author Dubery, IA
dc.date.accessioned 2018-07-09T08:23:59Z
dc.date.available 2018-07-09T08:23:59Z
dc.date.issued 2017-01
dc.identifier.citation Mhlongo, M.I. et al. 2017. Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. Biochemical and Biophysical Research Communications, vol. 482(4): 1498-1503 en_US
dc.identifier.issn 0006-291X
dc.identifier.issn 1090-2104
dc.identifier.uri https://www.sciencedirect.com/science/article/pii/S0006291X16321076
dc.identifier.uri doi.org/10.1016/j.bbrc.2016.12.063
dc.identifier.uri http://hdl.handle.net/10204/10291
dc.description Copyright: 2017 Elsevier. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. en_US
dc.description.abstract To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Worklist;20321
dc.subject Azelaic acid en_US
dc.subject Lipopolysaccharide en_US
dc.subject Metabolomics en_US
dc.subject Phytohormones en_US
dc.subject Salicylic acid en_US
dc.title Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells en_US
dc.type Article en_US
dc.identifier.apacitation Mhlongo, M., Tugizimana, F., Piater, L., Steenkamp, P. A., Madala, N., & Dubery, I. (2017). Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. http://hdl.handle.net/10204/10291 en_ZA
dc.identifier.chicagocitation Mhlongo, MI, F Tugizimana, LA Piater, Paul A Steenkamp, NE Madala, and IA Dubery "Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells." (2017) http://hdl.handle.net/10204/10291 en_ZA
dc.identifier.vancouvercitation Mhlongo M, Tugizimana F, Piater L, Steenkamp PA, Madala N, Dubery I. Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. 2017; http://hdl.handle.net/10204/10291. en_ZA
dc.identifier.ris TY - Article AU - Mhlongo, MI AU - Tugizimana, F AU - Piater, LA AU - Steenkamp, Paul A AU - Madala, NE AU - Dubery, IA AB - To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells. DA - 2017-01 DB - ResearchSpace DP - CSIR KW - Azelaic acid KW - Lipopolysaccharide KW - Metabolomics KW - Phytohormones KW - Salicylic acid LK - https://researchspace.csir.co.za PY - 2017 SM - 0006-291X SM - 1090-2104 T1 - Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells TI - Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells UR - http://hdl.handle.net/10204/10291 ER - en_ZA


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