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Browsing by Author "Roth, Robyn L"

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Analyzing the molecular mechanism of lipoprotein localization in Brucella

Author: Goolab, Shivani ; Roth, Robyn L ; Crampton, Michael C ; Van Heerden, H Date: Oct 2015 Bacterial lipoproteins possess diverse structure and functionality, ranging from bacterial physiology to pathogenic processes. As such many lipoproteins, originating from Brucella are exploited as potential vaccines to countermeasure brucellosis ... Read more
Cloning, multicopy expression and fed-batch production of Rhodotorula araucariae epoxide hydrolase in yarrowia lipolytica

Author: Ramduth, D ; Roth, Robyn L ; Lalloo, Rajesh ; Simpson, C ; Mitra, RK ; Gorgens, J ; Ramchuran, Santosh O ; Mathiba, K Date: May 2008 Epoxide hydrolases (EHs) of fungal origin have the ability to catalyse the enantioselective hydrolysis of epoxides to their corresponding diols. However wild type fungal EHs are limited in the substrate range and enantioselectivity, additionally ... Read more
Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197

Author: Roth, Robyn L ; Van Zyl, Petrus J ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mamputha, Sipho ; Buthelezi, Sindisiwe G ; Crampton, Michael C Date: Apr 2017 The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application ... Read more
Conserved phosphoryl transfer mechanisms within kinase families and the role of the C8 proton of ATP in the activation of phosphoryl transfer

Author: Kenyon, CP ; Roth, Robyn L ; Van der Westhuyzen, Christiaan W ; Parkinson, CJ Date: Mar 2012 The kinome is made up of a large number of functionally diverse enzymes, with the classification indicating very little about the extent of the conserved kinetic mechanisms associated with phosphoryl transfer. It has been demonstrated that ... Read more
Differential inhibition of adenylylated and deadenylylated forms of M. tuberculosis glutamine synthetase as a drug discovery platform

Author: Theron, Anjo ; Roth, Robyn L ; Hoppe, H ; Parkinson, C ; Van der Westhuyzen, Christiaan W ; Stoychev, Stoyan H ; Wiid, I ; Pietersen, RD ; Baker, B ; Kenyon, CP Date: Oct 2017 Glutamine synthetase is a ubiquitous central enzyme in nitrogen metabolism that is controlled by up to four regulatory mechanisms, including adenylylation of some or all of the twelve subunits by adenylyl transferase. It is considered a ... Read more
Heterologous expression of plasmodial proteins for structural studies and functional annotation

Author: Birkholtz, LM ; Blatch, G ; Coetzer, TL ; Hoppe, HC ; Human, E ; Morris, EJ ; Ngcete, Z ; Oldfield, LC ; Roth, Robyn L ; Shonhai, A ; Stephens, L ; Louw, AI Date: 2008 Malaria remains the world's most devastating tropical infectious disease with as many as 40% of the world population living in risk areas. The widespread resistance of plasmodium parasites to the cost-effective chloroquine and antifolates has ... Read more
Heterologous expression of trametes versicolor laccase in pichia pastoris and aspergillus niger

Author: Bohlin, C ; Jonsson, LJ ; Roth, Robyn L ; Van Zyl, WH Date: Sep 2006 Laccases are copper-containing phenol-oxidizing enzymes that are useful in many different applications, for example in lignocellulose processing and textile industry. Efficient and convenient systems for heterologous expression are needed for ... Read more
Ovarian function following immunocontraceptive vaccination of mares using native porcine and recombinant zona pellucida vaccines formulated with a non-Freund's adjuvant and anti-GnRH vaccine

Author: Nolan, MB ; Bertschinger, HJ ; Roth, Robyn L ; Crampton, Michael ; Martins, IS ; Fosgate, GT ; Stout, TA ; Schulman, ML Date: Oct 2018 An important determinant in the selection of any contraceptive agent is the impact on ovarian function, both in the short and longer term. In this study, ovarian activity was monitored in mares immunised with one of the following vaccine ... Read more
The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases

Author: Kenyon, CP ; Steyn, A ; Roth, Robyn L ; Steenkamp, PA ; Nkosi, TC ; Oldfield, LC Date: Jul 2011 Kinase and synthetase enzymes utilise C8D-ATP in preference to non-deuterated ATP. The KIE obtained at low ATP concentrations is clearly a primary KIE demonstrating strong evidence that the bond to the isotopically substituted hydrogen is ... Read more
Serum antibody immunoreactivity and safety of native porcine and recombinant zona pellucida vaccines formulated with a non-Freund’s adjuvant in horses

Author: Nolan, M ; Schulman, M ; Botha, A ; Human, A ; Roth, Robyn L ; Crampton, Michael C ; Bertschinger, H Date: Feb 2019 Commercial and regulatory limitations associated with native porcine zona pellucida (pZP) vaccines formulated with Freund’s adjuvants may be overcome by developing effective recombinant ZP vaccines (reZP) and identifying alternative adjuvant ... Read more

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Browsing by Author "Roth, Robyn L"

Browsing by Author "Roth, Robyn L"

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